水稻类病斑早衰突变体lmps1的表型鉴定与基因定位  

Phenotypic characterizing and gene mapping of a lesion mimic and premature senescence 1 (lmps1) mutant in rice (Oryza sativa L.)

作　　者：夏赛赛 崔玉 李凤菲 谭佳[1] 谢园华 桑贤春[1] 凌英华[1] XIA Sai-Sai;CUI Yu;LI Feng-Fei;TAN Jia;XIE Yuan-Hua;SANG Xian-Chun;LING Ying-Hua(Rice Research Institute of Southwest University / Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops,Chongqing 400715, China)

机构地区：[1]西南大学水稻研究所/转基因植物与安全控制重庆市重点实验室,重庆400715

出　　处：《作物学报》2019年第1期46-54,共9页Acta Agronomica Sinica

基　　金：重庆市社会事业与民生保障科技创新专项项目(cstc2016shms-ztcx80012);中央高校基本科研业务费专项资金(XDJK2016A013)资助~~

摘　　要：经甲基磺酸乙酯(EMS)诱变优良籼型水稻恢复系缙恢10号,获得一个稳定遗传的水稻类病斑早衰突变体lmps1(lesion mimic and premature senescence 1)。该突变体苗期表型正常,分蘖早期出现褐色类病斑,且斑点数目随植株生长而增多,孕穗期叶片开始萎黄衰老。与野生型相比,突变体lmps1的每穗总粒数下降8%(P<0.05),株高、穗长、有效穗数、每穗实粒数、结实率以及千粒重分别下降14.3%、24.3%、27.2%、50%、45.7%与14.5%,差异均达极显著水平(P<0.01)。遮光处理表明,突变体lmps1的类病斑性状受光照诱导。孕穗期叶片光合色素含量下降且光合效率降低, H2O2含量增加,抗氧化酶SOD和CAT的活性显著降低。透射电镜观察结果显示,突变体lmps1叶肉细胞中叶绿体数目减少,叶绿体的类囊体片层结构损伤降解。qRT-PCR结果显示,突变体lmps1中防卫反应相关基因除POX22.3表达量降低外,POC1、PAL、PBZ1、PR1、NPR1、PR5表达量均极显著高于野生型。遗传分析表明突变体lmps1的类病斑早衰性状受1对隐性核基因控制,利用西农1A与突变体lmps1杂交所得F2群体中的突变株,将目标基因定位于第7染色体长臂端粒附近约167.3 kb的物理区段内。An elite rice indica restorer line,Jinhui10was chemically mutated with ethyl methane sulfonate(EMS).A novel and stable mutant,lesion mimic and premature senescence1(lmps1)was identified from the descendant library.The growth and development of lmps1plant was normal at seedling stage,but its leaves began to exhibit brown spots at the beginning of tillering stage,and the number of the spots increased as the plant growing up.When mutant plant entered booting stage,leaves started to wilt and became yellowish,then exhibited premature senescence.Compared with wild type,grains per panicle of lmps1decreased by8%,significantly lower than that of wild type(P<0.05).Besides,plant height,panicle length,effective panicle number,filled grain number per panicle,seed setting rate,and1000-grain weight of lmps1correspondingly decreasedly14.3%,24.3%,27.2%,50.0%,45.7%,and14.5%,statistically lower than those of wild type(P<0.01).The shading treatment suggested that the mutant performance of lmps1was light induced.Within the cells of lmps1,both of photosynthetic pigment content and photosynthetic efficiency decreased.At the same time,H2O2content increased,and activities of protective enzymes,SOD and CAT,reduced in lmps1.The observation by transmission electronic microscope(TEM)demonstrated that chloroplast number reduced in lmps1,and the chloroplast thylakoid lamellar structure seriously damaged and degraded.As a result of qRT-PCR,the expression levels of POC1,PAL,PBZ1,PR1,NPR1,and PR5were higher in lmps1than in wild type,while that of POX22.3was lower than that of wild type.The genetic analysis indicated that the lesion mimic and premature senescence of lmps1was under the control of a recessive nuclear gene.Mapping result via F2population derived from the cross between Xinong1A and lmps1showed that,the target gene was located in a167.3kb physical interval near the telomere of the long arm of chromosome7in rice(Oryza sativa L.).

关 键 词：水稻(Oryza SATIVA L.) 类病斑早衰突变体 (lmps1) 表型鉴定 基因定位 

分 类 号：S435.11[农业科学—农业昆虫与害虫防治]