急性单核细胞白血病相关抗原基因MLAA-22全长的克隆及生物信息学分析  

Cloning of The Full-length of Acute Monocytic Leukemia Related Antigen Gene MLAA-22 and Bioinformatics Analysis of It

作　　者：古流芳[1] 张鹏宇[1] 曹星梅[1] 何爱丽[1] 张王刚[1] GU Liufang;ZHANG Pengyu;CAO Xingmei(The Second Affiliated Hospital of Xi'an Jiao Tong University,Xi'an,710004)

机构地区：[1]西安交通大学第二附属医院,710004

出　　处：《实用癌症杂志》2018年第12期1920-1923,1927,共5页The Practical Journal of Cancer

基　　金：国家自然科学基金(编号:H0812)

摘　　要：目的获得急性单核细胞白血病相关抗原基因MLAA-22cDNA全长序列,以便进行新基因的生物学功能研究。方法本研究采用cDNA末端快速扩增法(rapid-amplification of cDNA ends,RACE),从人急性单核细胞白血病细胞株U937中克隆获得了的cDNA全长,并对MLAA-22进行了生物信息学分析及预测。结果 MLAA-22 cDNA全长3067bp,染色体定位于17q11. 2,基因包含12个外显子和11个内含子。生物信息学预测其有完整的ORF框,编码1个含有701个氨基酸残基的蛋白质,蛋白分子量约80. 5 kD,非分泌型,亚细胞定位为胞质蛋白。大多数capase酶均对MLAA-22无剪切作用。新的MLAA-22序列的5'端序列1-349 bp与KIAA0100 mRNA无同源性,但这一段序列与人类基因组序列完全匹配,属于MLAA-22的5'UTR(untranslated regions,UTR)。结论 MLAA-22基因cDNA全长3067 bp,5'UTR可能发生了选择性剪接,推测MLAA-22可能是一个和急性单核细胞白血病相关的新的选择性剪接变体,它参与了急性单核细胞白血病的发生,值得进一步深入研究。Objective Background our group constructed U937 Cells Cloning cDNA expression library with the characteristics of mononuclear cells by SEREX technology to look for acute monocytic leukemia associated antigen(MLAA).MLAA-22is one of representative new genes.Because sequence of MLAA-22 were obtained from cDNA libraries,its sequence may not be full-length.Methods The full-length of MLAA-22 cDNA was successfully amplified by 3'-RACE and 5'-RACE,and MLAA-22 gene and protein was analyzed by Bioinformatics.Results The sequence analysis after RACE revealed that the full-length of MLAA-22 was 3067bp.Compared with the original sequence of MLAA-22,the new sequence extended for 424 bp and 606 bp at 5'and 3'ends separately.MLAA-22 is located in 17q11.2 and it is highly homologous to human putative genes KIAA0100.Conclusion The full-legth of MLAA-22 cDNA 3067 bp.It could be a selective splice variant related to pathogenesis of the acute monocytic leukemia,which deserved to be studied in depth.

关 键 词：急性单核细胞白血病 MLAA-22 RACE CDNA全长 生物信息学分析 

分 类 号：R733.71[医药卫生—肿瘤]