形态学、流式细胞术以及免疫组化在检测多发性骨髓瘤患者中的应用效果  

Morphology,Flow Cytometry and Immunohistochemistry in the Detection of Multiple Myeloma Patients

作　　者：王慧[1] 高洁[2] WANG Hui;GAO Jie(Baoji Hospital of TCM,Baoji,710061)

机构地区：[1]陕西省宝鸡市中医医院,710061 [2]陕西省西安市第一医院,710002

出　　处：《实用癌症杂志》2018年第12期1963-1966,共4页The Practical Journal of Cancer

摘　　要：目的研究形态学、流式细胞术以及免疫组化在检测多发性骨髓瘤患者中的应用效果。方法选择60例多发性骨髓瘤患者,均按照常规方法进行骨髓穿刺,并进行形态学、流式细胞术以及免疫组化检测。观察形态学检查、流式细胞学检测以及免疫组化检测的结果。结果 60例多发性骨髓瘤患者均出现不同程度的免疫球蛋白增加和正色素性贫血,骨髓细胞形态学结果主要表现为异常浆细胞增生,且出现质的改变。流式细胞学检测CD45表达异常(55例阴性、5例部分阳性); CD19的阴性表达率为100. 00%; CD38以及CD138的阳性表达率均为100. 00%。免疫组化检测Kappa阳性43例,CD138阳性表达率为100. 00%,lambda阳性16例。流式细胞学以及免疫组化检测的阳性诊断符合率相比无明显差异(P> 0. 05);形态学检查骨髓瘤细胞所占比例明显高于流式细胞学以及免疫组化检测(P <0. 05)。结论形态学、流式细胞术以及免疫组化在检测多发性骨髓瘤患者中均具有较高的应用价值,可按照患者的具体情况,将三者联合使用以提高临床阳性诊断率和疗效监测。Objective To investigate morphology,flow cytometry and immunohistochemistry in the detection of multiple myeloma patients.Methods Selected 60 cases of patients with multiple myelomawho were treated.The bone marrow biopsy was carried out according to the routine method,and the morphology,flow cytometry and immunohistochemistry were performed.Results 60 cases of multiple myeloma patients in this study were immune globulin increased in different degree and normochromic anemia,bone marrow cell morphology results showed abnormal plasma cell hyperplasia,and the emergence of a qualitative change;The expression of CD45 was abnormal in flow cytometry(55 negative,5 partial positive),the negative expression rate of CD19 was 100.00%,and the positive rate of CD38 and CD138 was 100.00%;43 cases of Kappa positive were detected by immunohistochemistry,the positive rate of CD138 expression was 100.00%,and 16 cases were lambda positive;there was no significant difference in the rate of positive diagnosis between flow cytometry and immunohistochemistry(P>0.05);the proportion of myeloma cells in morphological examination was higher than that in flow cytometry and immunohistochemistry(P<0.05).Conclusion The morphology,flow cytometry and immunohistochemical detection has higher application value in multiple myeloma patients,according to the specific circumstances of the patients,the combined use of the three to improve the monitoring of clinical diagnosis rate and curative effect.

关 键 词：形态学 流式细胞术 免疫组化 多发性骨髓瘤 

分 类 号：R733.3[医药卫生—肿瘤]