儿茶素对软脂酸导致的大鼠原代肝细胞损伤的保护作用  

Protective effect of catechinic acid against palmitic acid induced endoplasmic reticulum stress-mediated apoptosis in primary rat hepatocytes

作　　者：苗良生 王晓娟 关新江 李万鹏 董小章 倪秀梅 李雪[3] 张勇[3] MIAO Liang-sheng;WANG Xiao-juan;GUAN Xin-jiang;LI Wan-peng;DONG Xiao-chang;NI Xiu-mei;LI Xue;ZHANG Yong(Department of Anesthesia, Weinan Central Hospital, Weinan 714000 , China;Department of Anesthesiology ,Weinan Maternal and Child Health Hospital ,Weinan 714000 , China;Department of Anesthesia, Second Affiliated Hospital of Xiran Jiaotong University ,Xiran 710004 , China)

机构地区：[1]渭南市中心医院麻醉手术科,陕西渭南714000 [2]渭南市妇幼保健院麻醉手术科,陕西渭南714000 [3]西安交通大学第二附属医院麻醉手术科,陕西西安710004

出　　处：《延安大学学报（医学科学版）》2018年第4期6-9,共4页Journal of Yan'an University:Medical Science Edition

基　　金：陕西省自然科学基础研究计划(NO:2014JM2-3034);西安交通大学第二附属医院基金青年项目[NO:YJ(QN)201301];陕西省渭南市科研发展计划项目(社发攻关)(NO:2015KYS-1-1)

摘　　要：目的探讨儿茶素能否对软脂酸所导致的大鼠原代肝细胞毒性具有保护作用。方法使用大鼠原代肝细胞分别与毒胡萝卜素和软脂酸以及儿茶素共孵育16 h后检测内质网应激相关蛋白GRP78、GRP94和CHOP,同时检测细胞凋亡和坏死。结果软脂酸与毒胡萝卜素类似,均可使共孵育的大鼠原代肝细胞GRP78、GRP94和CHOP的水平升高,而儿茶素的加入可以减少细胞凋亡和坏死并显著减少这三种标志物的产生(P <0. 05)。结论儿茶素可经由抑制内质网应激从而保护细胞不受软脂酸的毒性影响。Objective To study the ability of catechinic acid to prevent endoplasmic reticulum stress-mediated apoptosis of rat hepatocytes elicited by two compounds,i.e.thapsigargin and palmitic acid.Palmitic acid induced endoplasmic reticulum(ER)stress and caused apoptotic and necrotic cell death in the primary rat hepatocytes.Methods Incubation of primary rat hepatocytes for16h with palmitic acid produced a significant increase in cell death.Palmitic acid also increased levels of three indicators of ER stress-glucose regulated protein78(GRP78),C/EBP homologous protein(CHOP),and glucose regulated protein94(GRP94).Results Catechinic acid distinctly reduced cell death and levels of all three indicators of ER stress brought about by palmitic acid.Thapsigargin,which induces ER stress produced similar effects to those obtained using palmitic acid;its effects were partly reversed by catechinic acid,P<0.05.Conclusion These results suggest that catechinic acid prevents ER stress-mediated apoptosis of palmitic acid in primary rat hepatocytes.

关 键 词：儿茶素 软脂酸 内质网应激 

分 类 号：R285[医药卫生—中药学]