姜黄素预处理对干热环境热射病大鼠心肌氧化应激及细胞凋亡的影响  

Effects of curcumin pretreatment on the oxidative stress and apoptosis of cardiomyocytes in heatstroke rats in a dry heat environment

作　　者：马娜[1] 李佳佳[1] 宋来阳[1] 董翔[1] 康燕 张东辉[1] 李建瑛[1] 许琴[1] 是文辉[1] 刘江伟[1] MA Na;LI Jiajia;SONG Laiyang;DONG Xiang;KANG Yan;ZHANG Donghui;LI Jianying;XU Qin;SHI wenhui;LIU Jiangwei(Key Laboratory of Special Environmental Medicine of Xinjiang,General Hospital of Xinjiang Military Command,Urumqi 830000,China;No. 69240 Army Hospital, Urumqi, 830000)

机构地区：[1]新疆军区总医院新疆特殊环境医学重点实验室 [2]解放军69240部队医院

出　　处：《中国比较医学杂志》2018年第12期14-18,共5页Chinese Journal of Comparative Medicine

基　　金：自治区自然科学基金项目(2015211C231);军队临床高新技术重大项目(2010gxjs016)

摘　　要：目的观察姜黄素预处理对干热环境热射病大鼠心肌氧化应激及细胞凋亡的影响并探讨其可能机制。方法 50只SD雄性大鼠随机分为5组(n=10):常温对照组(NC),干热对照组(DHC),姜黄素50 mg/kg预处理组(L-cur),姜黄素100 mg/kg(M-cur),姜黄素200 mg/kg(H-cur)。NC、DHC组给予生理盐水灌胃,姜黄素预处理组给予不同浓度的姜黄素灌胃,每天1次,连续7天。第8天除NC组外,其余4组大鼠转移至西北特殊环境人工实验舱内进行实验,环境温度(41±0. 5)℃,湿度(10±1)%。实验的第150分钟达到热射病状态,麻醉后取材,用全自动生化检测仪检测大鼠血清肌酸激酶(CK)和乳酸脱氢酶(LDH)的变化,用TUNEL法检测心肌细胞凋亡并计算心肌细胞凋亡率。检测心肌超氧化物歧化酶(SOD)、丙二醛(MAD)的含量,并进行相关性分析。结果干热对照组大鼠血清心肌酶CK、LDH、心肌细胞凋亡指数、MDA较常温对照组显著升高(P <0. 01),姜黄素预处理组CK、LDH、心肌细胞凋亡指数、MDA均随药物浓度增加而降低,姜黄素各个预处理组之间差异具有显著性(P <0. 01); SOD的变化趋势与MDA相反。相关性分析提示,心肌细胞凋亡与大鼠血清心肌酶CK、LDH之间存在正相关性(相关系数分别为r=0. 302,r=0. 304,P <0. 05);心肌细胞凋亡与MDA之间存在正相关性(相关系数为r=0. 916,P <0. 01);心肌细胞凋亡与SOD之间存在负相关性(系数为r=-0. 863,P <0. 01)。结论姜黄素预处理对干热环境热射病大鼠心肌损伤具有保护作用,姜黄素可能通过抗氧化应激、减少细胞凋亡来发挥心肌损伤保护作用。Objective To observe the effects of curcumin pretreatment on myocardial oxidative stress and apoptosis in heatstroke rats in a dry heat environment and its possible mechanism.Methods Fifty male Sprague-Dawley rats were randomly divided into five groups(n=10):normal control group(NC),dry and control group(DHC),and50mg/kg curcumin(L-cur),100mg/kg curcumin(M-cur),and200mg/kg curcumin(H-cur)pretreatment groups.NC and DHC groups were gavaged with saline,and curcumin pretreatment groups were gavaged with the various concentrations of curcumin once daily for7days.On day8,except in the NC group,the rats were transferred to a climate cabin(Simulated Climate Cabin for Special Environment of Northwest of China)under the conditions of40.5±0.5°C and10±1%relative humidity for150min to establish the heatstroke model of a dry heat environment.Then,the rats were anaesthetized and sacrificed at150min,and blood and heart tissues were collected.Serum myocardial enzyme creatine kinase(CK)and lactate dehydrogenase(LDH)were detected by an automatic biochemical detector.Myocardial cell apoptosis was detected by TUNEL method,and the apoptosis index was calculated.Myocardial superoxide dismutase(SOD)and malonaldehyde(MAD)were detected with SOD and MDA kits,respectively.Results Serum CK,LDH,cardiomyocyte apoptosis index,and MDA were significantly higher in the DHC group than in the NC group(P<0.01).CK,LDH,cardiomyocyte apoptosis index,and MDA of curcumin pretreatment groups were decreased with the increase in curcumin concentration,and there was a significant different among the curcumin pretreatment groups(P<0.01).However,SOD showed an opposite trend to MDA.Correlation analysis indicated a positive correlation between the myocardial apoptosis index and serum CK and LDH in rats(r=0.302,r=0.304,P<0.05).There was a positive correlation between the myocardial apoptosis index and MDA(r=0.916,P<0.01).However,there was a negative correlation with SOD(r=-0.863,P<0.01).Conclusions Curcumin pretreatment has a protective effect on myocardial inju

关 键 词：姜黄素 干热环境 热射病 大鼠 心肌 凋亡 氧化应激 

分 类 号：R-33[医药卫生]