不同林型、产地、参龄及坡向对林下参20种单体皂苷含量的影响  

Effects of Different Forest Types,Producing Areas,Ages and Slope Orientations on Contents of 20 Ginsenosides in Roots of Panax ginseng C.A. Meyer cv. Silvatica

作　　者：阮晓佳 郜玉钢[1] 赵岩[1] 臧埔 何忠梅[1] 祝洪艳[1] 王立岩[1] 张连学[1] RUAN Xiaojia;GAO Yugang;ZHAO Yan;ZANG Bu;HE Zhongmei;ZHU Hongyan;WANG Liyan;ZHANG Lianxue(College of Traditional Chinese Medicine, Jilin Agricultural University, Changchun 130118, China)

机构地区：[1]吉林农业大学中药材学院,吉林长春130118

出　　处：《食品科学》2018年第24期195-202,共8页Food Science

基　　金："十三五"国家重点研发计划重点专项(2016YFC0500300);吉林省科技厅项目(20160307005YY;20170204017YY;2015N024)

摘　　要：采用同时测定20种人参皂苷高效液相色谱法评价不同林型、产地、参龄及坡向对林下参皂苷含量的影响。结果表明,不同林型、产地、参龄及坡向林下参均含有14种人参皂苷单体;与其他3种林型比较,樟子松林型下栽培的林下参单体皂苷Rg1、Re、Rf、Rb1、Rb2、Rb3、Rg5含量与20种单体皂苷加和值最高;与其他5个产地相比,抚松县露水河镇林下参单体皂苷Rg1、Rf含量最高;3种不同年生林下参比较,15 a生林下参单体皂苷Rg1、Rf、Rb1含量最高,5 a生林下参单体皂苷Compound K、原人参二醇含量最高;3种不同坡向相比,阳坡林下参单体皂苷Rb2、Rb3含量最高,阴坡林下参单体皂苷Rh2含量最高。不同林型、产地、参龄及坡向林下参皂苷种类相同但含量不同,5～15 a生林下参均达到2015年《中国药典》要求。Objective:To provide a scientific basis for the development and utilization of the roots of Panax ginseng C.A.Meyer cv.Silvatica.Methods:The effects of different forest types,producing areas,ages and slope orientations on the contents of ginsenosides in the roots of P.ginseng C.A.Meyer cv.Silvatica.were evaluated.Simultaneous determination of20ginsenosides was performed by high performance liquid chromatography(HPLC).Results:A total of14ginsenosides were detected in each sample regardless of forest types,producing areas,ages or slope orientations.The contents of ginsenosides Rg1,Re,Rf,Rb1,Rb2,Rb3and Rg5and the total content of20ginsenosides in the samples grown in the forest of Pinus sylvestris var.Mongolica were the highest among four forest types investigated.The contents of Rg1and Rf in the samples from Lushuihe township,Fusong county were the highest among six producing areas.Among three different ages,the contents of Rg1,Rf and Rb1of the15-year-old samples were the highest,while the contents of Compound K and protopanaxadiol(PPD)were the highest in the5-year-old samples.Among three different slope orientations,the contents of Rb2and Rb3in the samples from south-facing slope were the highest,while the content of Rh2in the samples from north-facing slope was the highest.Conclusion:The contents but not types of ginsenosides in the roots of P.ginseng C.A.Meyer cv.Silvatica varied with different forest types,producing areas,ages and slope orientations.The roots of P.ginseng C.A.Meyer cv.Silvatica aged5to15years could meet the requirements of Chinese Pharmacopoeia(CP,2015version).

关 键 词：林下参 人参皂苷 质量评价 高效液相色谱 

分 类 号：R917[医药卫生—药物分析学]