明永冰川低温细菌分离鉴定及生物学特性  

Isolation,Identification and Characterization of Cold-Adapted Bacteria from Mingyong Glacier

作　　者：赵晓曼 周长涛 魏云林[1] 季秀玲[1] ZHAO Xiaoman;ZHOU Changtao;WEI Yunlin;JI Xiuling(Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,China)

机构地区：[1]昆明理工大学生命科学与技术学院,云南昆明650500

出　　处：《食品与生物技术学报》2018年第12期1261-1265,共5页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(31700324;31860147)

摘　　要：对明永冰川冰舌地区的细菌进行分离培养和鉴定。通过土壤悬浮涂布法和平板划线法分离纯化细菌,并做生理生化特性、16S rRNA基因序列测定及系统发育分析。从明永冰川地区分离纯化出22株可培细菌,16S rRNA基因序列分析结果表明:菌株MY0504与黄杆菌属梭菌(Flavobacterium pectinovorum)亲缘关系最近(97.6%);菌株MY1413和MY14015分别与Arthrobacter psychrophenolicus和Arthrobacter parietes有很近的亲缘关系(98%);MY14011与河流色杆菌(Chromobacterium fluviatile)进化关系最近(97%),其余18株菌均鉴定为假单胞菌属的菌株(Pseudomonas spp.)。作者对该地区微生物资源进行初步探究,为了解冰川低温微生物多样性提供参考。We screened and identified the cold-adapted bacteria from Mingyong glacier.The bacterial strains were isolated and purified by the soil suspension coating and spread plate methods.Based on physiological and biochemical properties,homology and phylogenetic analysis of 16S rRNA gene sequences,all isolates were classified.In total 22 bacterial strains were isolated,based on the 16S rRNA gene sequence analysis:MY0504 strain was most closely related to Flavobacterium pectinovorum with 97.6%,MY1413 and MY14015 strains were most closely related to Arthrobacter psychrophenolicus and A.parietes with 98%,while MY14011 strain was most closely related to Chromobacterium fluviatile with 97%,and other 18 strains of bacteria were identified as Pseudomonas spp.The research is preliminary exploration on the microbiological resources in Mingyong glacier,and provides reference information for further studies in this area.

关 键 词：冰川 低温细菌 16S RRNA 分离鉴定 

分 类 号：Q938[生物学—微生物学]