高脂饮食诱导果蝇心衰模型的建立  

Establishment of a high-fat-diet-induced heart failure model in Drosophila melanogaster

作　　者：吴红梅 刘学文 文格波[2] 唐旻[1] WU Hongmei;LIU Xuewen;WEN Gebo;TANG Min(Institute of Biochemistry and Molecular Biology, University of South China, Hengyang 421001, China;the First Affiliated Hospital, University of South China, Hengyang 421001)

机构地区：[1]生物化学与分子生物学研究所,南华大学,湖南衡阳421001 [2]南华大学第一附属医院,湖南衡阳421001

出　　处：《中国实验动物学报》2018年第6期688-692,共5页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金(81500311,81711530050);湖南省科技厅课题(2015KJT01).

摘　　要：目的建立高脂饮食诱导的果蝇心衰模型。方法选择野生型果蝇w1118果蝇作为实验对象,采用含不同浓度椰油(0%,7.5%,15%,30%)食物喂食果蝇5 d,检测果蝇体内的三酰甘油含量。后续实验以非高脂喂食组(0%椰油)作为对照,30%椰油高脂喂食组作为实验组。负向趋地性实验检测果蝇运动能力。果蝇成体心脏功能分析平台,拍摄果蝇在半解剖条件下的心脏跳动情况(30 s),软件分析获得成体心脏功能指标,包括心跳周期,收缩和舒张直径,心脏收缩力指数等。实时定量PCR检测高脂喂食果蝇心脏的RNA表达变化。结果与非高脂喂食果蝇相比,不同比例高脂喂食果蝇的三酰甘油含量随椰油浓度呈剂量递增(P<0.05);高脂饮食显著影响果蝇活动力(P<0.001);高脂喂食果蝇的心跳周期缩短(P<0.01),心脏收缩力减弱(P<0.001);实时定量PCR发现高脂喂食果蝇中,参与脂代谢相关的一些基因fa2h,CG6277,CG3699,CG9914,lip2的表达明显下调(P<0.05)。结论高脂饮食严重影响果蝇运动能力和成体心脏功能,成功建立了高脂饮食诱导的果蝇心衰模型。Objective To establish a high-fat-diet-induced heart failure model in Drosophila melanogaster.Methods Wild-type Drosophila w1118was chosen as the experimental subject.The triglyceride level was examined in Drosophila fed with different concentrations of coconut oil(0%,7.5%,15%,30%).In the follow-up experiment,a high-fat diet containing30%coconut oil was used for the experimental group,in contrast to a non-fat diet(0%coconut oil)for a control group.Using Drosophila negative geotaxis assay,the physical activity was evaluated.The pulse was recorded for30s and analyzed.Adult heart physiological parameters,including heart period,systolic and diastolic diameters,as well as fractional shortening,were obtained.The RNA expression of metabolism-related genes was measured by real-time PCR.Results The triglyceride content in high-fat-diet-fed Drosophila increased gradually with the concentration of coconut oil(P<0.05).High-fat-diet-fed Drosophila showed dramatically decreased physical activity(P<0.001),as determined using the negative geotaxis assay,and compromised adult physiological heart function,characterized by a decreased heart period and fractional shortening(P<0.05).Metabolism-related genes,including fa2h,CG6277,CG3699,CG9914,and lip2,were downregulated in high-fat-diet-fed Drosophila compared with the levels in those with a non-fat diet.Conclusion The high-fat diet compromises physical activity and adult heart physiological function,revealing that a high-fat-diet-induced Drosophila heart failure model is successfully established here.

关 键 词：果蝇 心衰 高脂饮食 代谢 三酰甘油 

分 类 号：Q95-33[生物学—动物学]