Foxp3+Tregs及PD1在上皮性卵巢癌组织中的表达及其临床病理意义  

Expression and Clinicopathological Significance of Foxp3+Tregs and PD1 in Epithelial Ovarian Cancer

作　　者：张智[1] 张一琼[1] ZHANG Zhi;ZHANG Yi-qiong(Department of Obstetrics and Gynecology,the First Affiliated Hospital of Nanchang University,Nanchang 330006,China)

机构地区：[1]南昌大学第一附属医院妇产科,南昌330006

出　　处：《南昌大学学报（医学版）》2018年第5期54-58,69,共6页Journal of Nanchang University：Medical Sciences

基　　金：江西省卫生健康委员会普通科技计划项目(20175124)

摘　　要：目的研究Foxp3+调节性T细胞(Foxp3+Tregs)与程序性死亡受体1(PD1)在上皮性卵巢癌组织中的表达,以及两者与上皮性卵巢癌患者生存率的关系。方法 EnVision二步法检测21例正常卵巢组织、36例良性卵巢组织和52例上皮性卵巢癌组织中Foxp3+Tregs和PD1的表达水平;结合随访资料,分析卵巢癌组织中两者的表达与患者临床病理和预后的关系及两者的相关性。结果 Foxp3+Tregs和PD1在上皮性卵巢癌组织中的表达阳性率为57.69%、55.78%,两者在正常组织及卵巢良性组织中均未见表达。在上皮性卵巢癌组织中:Foxp3+Tregs的表达与FIGO分期及组织类型有关(P<0.05),与分化程度、淋巴转移、肿瘤大小无关(P>0.05);PD1的表达与FIGO分期及淋巴转移有关(P<0.05),与组织类型、分化程度、肿瘤大小无关(P>0.05);Foxp3+Tregs、PD1阴性患者较阳性患者生存率高(P<0.05);Foxp3+Tregs与PD1+TILs的表达呈正相关(r=0.381 7,P<0.05)。结论上皮性卵巢癌组织中可能存在Foxp3+Tregs和PD1的共同表达,两者共同检测有望成为上皮性卵巢癌患者特异性标志物。Objective To investigate the expression of Foxp3+regulatory T cells(Foxp3+Tregs)and programmed death receptor1(PD1)in epithelial ovarian cancer,and to analyze their relationships to survival in ovarian cancer patients.Methods The EnVision two-step method was used to detect the expression of Foxp3+Tregs and PD1in21normal ovarian tissues,36benign ovarian tissues and52ovarian cancer tissues.Furthermore,follow-up data were reviewed to analyze the relationships of Foxp3+Tregs and PD1expression to clinical pathology and prognosis.Results The positive rates of Foxp3+Tregs and PD1were57.69%and55.78%in ovarian cancer tissues,respectively.No Foxp3+Tregs and PD1expression were found in both normal tissues and benign ovarian tissues.The expression of Foxp3+Tregs was related to FIGO stage and tissue type(P<0.05),but was not related to differentiation degree,lymphatic metastasis and tumor size(P>0.05).The expression of PD1was correlated with FIGO stage and lymph node metastasis(P<0.05),but was not correlated with histological type,differentiation degree and tumor size(P>0.05).The survival rates in Foxp3+Tregs-negative patients and PD1-negative patients were higher than those in Foxp3+Tregs-positive patients and PD1-positive patients,respectively(P<0.05).There was a positive correlation between Foxp3+Tregs and PD1+TILs in ovarian cancer tissues(r=0.3817,P<0.05).Conclusion There is co-expression of PD1and Foxp3+Tregs in epithelial ovarian cancer,which may be specific markers for epithelial ovarian cancer.

关 键 词：上皮性卵巢癌 FOXP3+调节性T细胞 PD1 临床病理 表达 预后 

分 类 号：R737.31[医药卫生—肿瘤]