miR-1254在结肠癌中调控差异表达基因及信号通路研究  

Differentially expressed genes and signal pathways regulated by miR-1254 in colon cancer

作　　者：韩雨彤 董小燕 彭海霞[2] 褚以忞[2] 周锋利[2] 杨大明[2] 徐莹[2] 孙伟杰 陈曦 HAN Yu-tong;DONG Xiao-yan;PENG Hai-xia;ZHOU Feng-li;ZHOU Feng-li;YANG Da-ming;XU Ying;SUN Wei-jie;CHEN Xi(Department of Pathogenic Biology,Beihua University School of Medicine,Jilin 132013,China)

机构地区：[1]北华大学医学院病原生物学教研室,吉林吉林132013 [2]上海交通大学医学院附属同仁医院内窥镜室,上海200336

出　　处：《临床军医杂志》2018年第12期1398-1401,共4页Clinical Journal of Medical Officers

基　　金：上海市卫计委面上项目(201440426);上海市卫计委青年课题(20154Y0160);上海市卫计委青年课题(20174Y0084)

摘　　要：目的探讨miR-1254对结肠癌发生发展的影响及其在结肠癌中调控的差异表达基因(DEG)和信号通路。方法在人结肠癌细胞系SW1116中瞬转miR-1254模拟体,通过高通量RNA测序分析(RNA-seq),筛选出受miR-1254调控的DEG,由生物信息学分析这些DEG所涉及的信号通路和蛋白-蛋白相互作用(PPI)网络。结果通过RNA-seq,筛选出了217条DEG。其中,121条下调基因,96条上调基因。这些基因主要富集在p53信号通路、氨酰-tRNA生物合成和内吞、甘氨酸/丝氨酸/苏氨酸代谢以及N-聚糖生物合成等信号通路中。PPI网络显示,结合蛋白较多的5个中心节点蛋白为UMPS、EGR1、CDKN1A、YARS以及ASNS。结论 miR-1254是一个多功能miRNA,在结肠癌发生发展中起重要作用。Objective To investigate the effects of miR-1254 on the occurrence and development of colon cancer and the differentially expressed genes(DEG)and signaling pathways regulated in colon cancer.Methods In the human colon cancer cell line SW1116,the mimics of miR-1254 were transiently transfected through high-throughput RNA sequencing analysis(RNA-seq)to screen out the DEG regulated by miR-1254,and the signaling pathways and protein-protein interaction(PPI)networks involved in these DEGs were analyzed by bioinformatics.Results The RNA-seq results showed 217 DEGs,including 121 down-regulated genes and 96 up-regulated ones.According to the bioinformatics,DEGs were enriched in p53 signaling pathway,aminoacyl-tRNA biosynthesis,endocytosis,N-glycan biosynthesis and glycine,serine and threonine metabolism.PPI was analyzed to find the top 5 hub nodes including UMPS,EGR1,CDKN1A,YARS and ASNS.Conclusion The miR-1254 is a multifunctional miRNA that plays an important role in the development of colon cancer.

关 键 词：miR-1254 结肠癌 高通量RNA测序分析 

分 类 号：R735.3[医药卫生—肿瘤]