豌豆根瘤菌gshR基因的抗氧化和共生固氮作用  

Antioxidation and Symbiotic Nitrogen Fixation of gshR Gene in Rhizobium leguminosarum

作　　者：程国军[1] 谢婧 殷杰[1] 彭杨[1] Cheng Guojun;Xie Jing;Yin Jie;Peng Yang(College of Life Sciences,South-Central University for Nationlities,Wuhan 430074,China)

机构地区：[1]中南民族大学生命科学学院,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2018年第4期31-34,共4页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：湖北省自然科学基金资助项目(2016CFC771);中央高校基本科研业务费专项资金资助项目(CZW15112)

摘　　要：目的:研究豌豆根瘤菌RL3841的谷胱甘肽还原酶编码基因gshR的功能.方法:采用基因同源重组构建了豌豆根瘤菌gshR基因突变株RLgshR,探讨了基因突变对根瘤菌抗氧化和共生固氮的影响,利用实时荧光定量RTPCR检测gshR基因的mRNA表达水平.结果:gshR基因缺失不影响菌株在AMS基础培养基中的生长能力,但突变株对氢过氧化枯(CuOOH)和较高浓度的H_2O_2氧化物敏感.结论:gshR基因突变株RLgshR形成正常固氮根瘤,gshR基因的表达不受H_2O_2和共生环境诱导.Objective:To study the function of glutathione reductase gene gshR in rhizobium leguminosarum.Method:gshR mutant RLgshR was constructed through homologous recombination,and the effect of gene mutation on its symbiosis nitrogen fixation and antioxidative function were explored.Quantitative real-time RT-PCR was performed to detect mRNA expression of gshR gene.Results:The growth ability of RLgshR in AMS basal medium was not affected by the lack of gshR gene;however,the mutant strain was sensitive to CuOOH and high concentrations of H 2 O 2.Conclusion:The gshR mutant RLgshR formed nodules with normal nitrogen-fixing activity and gshR gene expression can not be induced under H 2 O 2 stress and symbiotic condition.

关 键 词：豌豆根瘤菌 gshR基因 抗氧化功能 共生固氮 荧光定量RT-PCR 

分 类 号：Q935[生物学—微生物学]