淫羊藿苷通过VEGF通路对缺氧诱导的脉络膜视网膜内皮细胞系RF/6A增殖、侵袭和迁移能力的调节作用  

Icariin regulates proliferation,invasion and migration of hypoxia-induced choroid retinal endothelial cells RF/6A

作　　者：刁婷婷 田骋 王洪亮 姜玉珍 DIAO Ting-Ting;TIAN Cheng;WANG Hong-Liang;JIANG Yu-Zhen(The First Affiliated Hospital of Nanyang Medical College,Nanyang 473000,China)

机构地区：[1]南阳医专第一附属医院,南阳473000

出　　处：《中国免疫学杂志》2018年第12期1819-1823,共5页Chinese Journal of Immunology

基　　金：河南省医学科技公关计划项目(No.201207128)

摘　　要：目的:探索淫羊藿苷对缺氧诱导的脉络膜视网膜内皮细胞RF/6A增殖、侵袭和迁移能力的影响及其机制。方法:MTT检测细胞增殖;Transwell检测细胞侵袭;划痕实验检测细胞迁移;蛋白印迹检测VEGF、p-PI3K、p-AKT和Rac蛋白水平。结果:与对照组相比,缺氧组细胞增殖明显升高,侵袭和迁移能力明显增强(P<0. 05)。淫羊藿苷处理缺氧组细胞后,细胞增殖能力明显下降,侵袭和迁移能力明显减弱(P<0. 05)。而且,缺氧组VEGF、p-PI3K、p-AKT和Rac蛋白水平显著高于正常组(P<0. 01)。与缺氧组相比,缺氧加药组VEGF、p-PI3K、p-AKT和Rac蛋白水平明显降低(P<0. 01)。结论:淫羊藿苷通过VEGF通路抑制缺氧诱导的脉络膜视网膜内皮细胞RF/6A增殖、侵袭和迁移能力。Objective:To explore the effect of icariin on the proliferation,invasion and migration of hypoxia-induced choroid retinal endothelial cells RF/6A.Methods:Cell proliferation was measured by MTT.Invasion was detected via Transwell.Migration was tested through wound healing.The expression of VEGF,p-PI3K,p-AKT and Rac was detected by Western blot.Results:Compared with control group,the proliferation,invasion and migration in hypoxia group were elevated(P<0.05).After treatment with icariin,the proliferation,invasion and migration of hypoxia-induced RF/6A cells were reduced(P<0.05).Moreover,the protein levels of VEGF,p-PI3K,p-AKT and Rac in hypoxia group were higher than control group(P<0.01).Conclusion:Icariin inhibits the proliferation,invasion and migration of hypoxia-induced RF/6A cells via VEGF pathway.

关 键 词：淫羊藿苷 增殖 侵袭 迁移 VEGF通路 

分 类 号：R774.13[医药卫生—眼科]