苹果全基因组PAL基因家族成员的鉴定及表达分析  

Identification and expression analysis of PAL gene family in apple

作　　者：张丽之 樊胜[1] 安娜[1] 左希亚 高彩 张东[1] 韩明玉[1] ZHANG Lizhi;FAN Sheng;AN Na;ZUO Xiya;GAO Cai;ZHANG Dong;HAN Mingyu(College of Horticulture,Northwest A&F University,Yangling 712100,China)

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《浙江农业学报》2018年第12期2031-2043,共13页Acta Agriculturae Zhejiangensis

基　　金：国家现代农业产业技术体系(CARS-27);陕西省科技厅项目(2016KTZDNY01-10;2017NY0055)

摘　　要：根据金冠苹果参考基因组,利用BLAST在线网站鉴定得到苹果基因组中共8个PAL基因,所有MdPAL蛋白都含有MIO保守结构域(Ala-Gly-Ser)。分组鉴定和进化树分析结果显示,MdPAL蛋白可以被分为3组,分子量在47. 713～81. 748 ku,等电点在5. 44～6. 39,进化关系较近的成员拥有相似的基因结构和蛋白保守基序分布。利用GEO数据库和实时荧光定量PCR的方法检测苹果长富2号品种中PAL基因在不同组织器官及5个不同果实发育阶段的表达情况。不同组织器官表达结果表明,MdPAL2、MdPAL3/4/6/7、MdPAL8在叶中表达最高,而MdPAL1和MdPAL5在花中表达最高,表达模式存在多样性。不同果实发育阶段表达结果发现,MdPAL表达规律不同,所有MdPAL在幼果发育期或果实成熟期显著升高(除MdPAL1外),暗示其在果实发育和成熟过程具有重要作用。Based on apple genome and the genomic database of Golden Delicious,8 apple PAL genes were identified through BLAST tool.All members shared conserved MIO active site including Ala-Gly-Ser.The analysis of classification and evolutionary relationship showed that the 8 MdPAL genes were divided into three groups,the molecular weight was between 47.713 and 81.748 ku,the isoelectric point was between 5.44 and 6.39,and the members of one subgroup shared similar gene structure and conserved protein motifs.Based on GEO database and qRT-PCR,expression patterns of MdPAL genes in different organs and different stages of fruits in Nagafu No.2 were determined.The analysis of expression patterns of MdPAL genes in different organs showed that MdPAL2,MdPAL3/4/6/7,MdPAL 8 had the highest expression levels in leaves,while MdPAL 1 and MdPAL 5 expressions were the highest in flowers.The expression patterns were diverse.In 5 stages of the fruit development in Nagafu No.2,the expression patterns of MdPAL genes were significantly increased in the young stage or the ripening stage except MdPAL 1,indicating that they might play important roles in different stages during the fruit development.

关 键 词：苹果 PAL基因家族 生物信息学 系统进化分析 基因表达 

分 类 号：S661.1[农业科学—果树学]