MLH1、MSH2基因多态性与肾癌发病风险的相关性研究  

MLH1 and MSH2 Gene Polymorphism and Correlation with Risk of Renal Cancer

作　　者：邢兆辉[1] 徐洋[1] 李红松[1] 李成镇[1] 王英慧[1] 牛吉瑞[1] XING Zhao-Hui;XU Yang;LI Hong-Song(Heilongjiang Provincial Hospital,Harbin, 150036,China)

机构地区：[1]黑龙江省医院,哈尔滨150036

出　　处：《黑龙江医学》2018年第11期1152-1154,共3页Heilongjiang Medical Journal

基　　金：黑龙江卫生厅科研课题(2016-514)

摘　　要：目的研究MLH1、MSH2基因多态性与肾癌发病风险的相关性。方法采用病例对照研究,应用PCR-RFLP法对180例肾癌病人及199名健康人的外周血进行MLH1 IVS14-19A> G和MSH2 c2063 T> G多态性位点基因分型。通过非条件logistic回归分析分别计算两组等位基因及基因型频率,通过年龄、性别校正后的比值比(OR)及其95%可信区间(95%CI)评估各种基因型与肾癌发病风险的相关性。结果 MLH1GG等位基因的携带者发生肾癌的风险是AA野生型纯合子的2. 42倍,MSH2突变杂合子TG和纯合子GG与野生型TT携带者相比,更易患肾癌。结论 DNA修复基因的两个多态性位点MSH2 c2063G突变等位基因及MLH1 IVS14-19G突变等位基因与肾癌的发生有关系。Objective To evaluate MLH1and MSH2gene polymorphism and correlation with risk of renal cancer.Methods Through applying case control research and PCR-RFLP method,180patients of kidney cancer and199health people were genotyped through periphery blood,including two SNP MLH1IVS14-19A>G和MSH2c2063T>G.Through non-condition logistic regression analysis,allele gene and genotype frequency of two groups were accounted.Correlation between various genotypes and risk of renal cancer was assessed by age and sex adjusted odds ratio(OR)and its95%confidence interval(95%CI).Result Carriers of the MLH1GG allele were2.42times more likely to develop renal cancer than AA wild-type homozygotes.MSH2mutant heterozygous TG and homozygous GG were more susceptible to renal cancer than wild-type TT carriers.Conclusion The MSH2c2063G mutant allele and the MLH1IVS14-19G mutant allele of the DNA repair gene are associated with the occurrence of renal cell carcinoma.

关 键 词：DNA错配修复 MSH2基因 MLH1基因 肾癌 单核苷酸多态性 

分 类 号：R737.11[医药卫生—肿瘤]