喉癌癌组织Survivin基因表达水平与凋亡相关因子水平的关系  

Relationship between Survivin gene expression level and levels of apoptosis related factors in laryngeal carcinoma tissues

作　　者：牟基伟[1] 张美佳[1] 吴建 MU Jiwei;ZHANG Meijia;WU Jian(Department of Otolaryngology,First Affiliated Hospital of Jiamusi University,Jiamusi,Heilongjiang 154003,China)

机构地区：[1]佳木斯大学附属第一医院耳鼻喉科,黑龙江佳木斯154003

出　　处：《重庆医学》2018年第35期4463-4466,共4页Chongqing medicine

基　　金：黑龙江省卫生和计划生育委员会科研课题(2014-249)

摘　　要：目的研究喉癌癌组织Survivin基因表达水平与bcl-2、p53、caspase-3等凋亡相关因子水平的关系。方法选取佳木斯大学附属第一医院2013年1月至2018年1月126例喉癌患者手术切除的癌组织标本与100例声带息肉标本为研究对象,采用免疫组织化学法检测喉癌癌组织与声带息肉中Survivin、bcl-2、p53、caspase-3表达,采用实时荧光定量聚合酶链反应技术检测Survivin、bcl-2、p53、caspase-3表达量,分析Survivin基因表达与喉癌患者临床病理特征的关系,比较喉癌癌组织与声带息肉中Survivin、bcl-2、p53、caspase-3阳性率及表达量,对喉癌癌组织Survivin与bcl-2、p53、caspase-3基因表达及表达量进行相关性分析。结果 Survivin阳性组TNM分期Ⅲ～Ⅳ期、中低分化、淋巴结转移患者比例显著高于Survivin阴性组(P<0.05)。喉癌癌组织Survivin、bcl-2、p53阳性率分别为68.3%、58.7%、63.5%显著高于声带息肉的46.0%、38.0%、42.0%(P<0.05),caspase-3阳性率为41.3%显著低于声带息肉的75.0%(P<0.05)。经Spearson相关性分析,喉癌癌组织Survivin表达与bcl-2、p53表达呈正相关,与caspase-3表达呈负相关。喉癌癌组织Survivin、bcl-2、p53表达量显著高于声带息肉(P<0.05),caspase-3表达量显著低于声带息肉(P<0.05)。喉癌癌组织Survivin表达量与bcl-2、p53表达呈正相关,与caspase-3表达呈负相关。结论 Survivin基因与bcl-2、p53、caspase-3等凋亡相关因子存在一定相关性,可通过抑制细胞凋亡与促进细胞增殖,参与喉癌发生、发展。Objective To study the relationship between the Survivin gene expression level and levels of apoptosis related factors such as bcl-2,p53 and caspase-3 in laryngeal carcinoma tissues.Methods The carcinoma tissue specimens operatively resected in 126 cases of laryngeal carcinoma and 100 cases of vocal cord polyp specimen in the First Affiliated Hospital of Jiamusi University from January 2013 to January 2018 were selected as the study objects.The expressions of Survivin,bcl-2,p53 and caspase-3 in laryngeal carcinoma tissues and vocal cord polyp tissues were detected by adopting the immunohistochemical method.The expression levels of Survivin,bcl-2,p53 and caspase-3 was detected by real-time fluorescence quantitative polymerase chain reaction.The relationship between Survivin gene expression and clinicopathological characteristics in the patients with laryngeal carcinoma was analyzed.The positive rates and expression levels of Survivin,bcl-p53 and caspase-3 were compared between laryngeal carcinoma tissues and vocal cord polyp tissues.The correlation of Survivin with expression and expression quantities of bcl-2,p53 and caspase-3 gene in laryngeal carcinoma tissues was analyzed.Results The proportions of the patients with the TNM stageⅢ-Ⅳ,middle and low differentiation and lymph node metastasis in the Survivin positive group were significantly higher than those in the Survivin negative group(P<0.05).The positive rates of Survivin,bcl-2 and p53 in laryngeal carcinoma tissues were 68.3%,58.7%and 63.5%respectively,which were significantly higher than 46.0%,38.0%and 42.0%in the vocal cord polyp tissues(P<0.05).The positive rate of caspase-3 was significantly lower than that in vocal cord polyp tissues(41.3%vs.75.0%,P<0.05).The Spearson correlation analysis showed that the expression of Survivin in the laryngeal carcinoma tissues was positively correlated with the expression of bcl-2 and p53,and negatively correlated with the expression of caspase-3.The expression quantities of Survivin,bcl-2 and p53 in laryngeal car

关 键 词：喉肿瘤 免疫组织化学 细胞凋亡 SURVIVIN bcl-2 p53 半胱氨酸天冬氨酸蛋白酶3 

分 类 号：R739.65[医药卫生—肿瘤]