3种方法检测食品中单增李斯特菌能力验证结果分析  

Analysis of Proficiency Testing Results of Detecting L. Monocytogenes in Foods with Three Methods

作　　者：丁秀琼 杨劲 刘骁 郑俏慧 林锏锐 庞晓林 DING Xiuqiong;YANG Jin;LIU Xiao;ZHENG Qiaohui;LIN Jianrui;PANG Xiaolin(Inspection and Quarantine Technology Center,Zhanjing Entry-Exit Inspection and Quarantine Bureau,Zhanjiang,Guangdong,524000,China)

机构地区：[1]湛江出入境检验检疫局检验检疫技术中心,广东湛江524000

出　　处：《检验检疫学刊》2018年第6期15-18,共4页Journal of Inspection and Quarantine

摘　　要：为了确保检测结果准确,提高实验室检测能力,采用GB 4789.30-2016、miniVIDAS、实时荧光PCR3种方法同时检测能力验证样品中的单增李斯特菌,并对测试结果进行比对分析。结果显示,所采用的3种方法均能准确鉴定出目标菌,取得满意结果。3种方法各有优劣,同时使用,综合判断,能确保试验结果准确。To ensure the accuracy of detection result and improve the detection capability of laboratory,three methods GB 4789.30-2016,mini VIDAS and Real-time PCR were employed for testing L.monocytogenes in the proficiency testing samples and the results were comparative analyzed.Result showed that the target bacteria could be identified accurately with the three methods and the test have achieved satisfactory result.The combination and synthetic judgment of the three methods can ensure the accuracy of the testing result while each have advantages and disadvantages.

关 键 词：GB 4789.30-2016 MINI VIDAS 实时荧光PCR 单增李斯特菌 能力验证 

分 类 号：Q93-332[生物学—微生物学]