结肠镜活检组织检测MGMT和galectin-3的表达在结肠腺癌中的临床意义  

Expression and the clinical significances of MGMT and galectin-3 in coloscopic biopsy specimens in colon adenocarcinoma

作　　者：苏赓[1] 梁科庆[1] 罗显克[2] 温伟[1] SU Geng;LIANG Keqing;LUO Xianke;WEN Wei(Department of Pathology,the Affiliated National Hospital of Guangxi Medical University Minzu Hospital of Guangxi Zhuang Autonomous Region,Guangxi Zhuang Autonomous Region,Nanning 530001,China;Department of Gastroenterology,the Affiliated National Hospital of Guangxi Medical University Minzu Hospital of Guangxi Zhuang Autonomous Region,Guangxi Zhuang Autonomous Region,Nanning 530001,China)

机构地区：[1]广西医科大学附属民族医院暨广西壮族自治区民族医院病理科,广西南宁530001 [2]广西医科大学附属民族医院暨广西壮族自治区民族医院消化内科,广西南宁530001

出　　处：《中国医药导报》2018年第35期94-97,109,183,共6页China Medical Herald

基　　金：广西壮族自治区卫生和计划生育委员会自筹经费科研课题(Z2015111)

摘　　要：目的检测结肠腺癌活检组织中DNA修复酶O^6-甲基鸟嘌呤-DNA甲基转移酶(MGMT)和半乳糖凝集素-3(galectin-3)的表达在结肠腺癌中的临床意义。方法选取2010年1月～2015年1月广西壮族自治区民族医院结肠镜活检的120例患者标本组织(包括60例结肠腺癌、30例炎性结肠黏膜病变和30例不典型增生结肠黏膜病变),通过免疫组化SP方法检测MGMT蛋白、galectin-3蛋白的表达情况,并通过随访了解预后情况。结果与炎性肠黏膜病变组比较,结肠腺癌组MGMT阳性表达降低,galectin-3阳性表达升高,差异有统计学意义(P <0.05)。与中-高分化结肠腺癌组比较,低分化结肠腺癌组中MGMT阳性表达降低,galectin-3阳性表达升高,差异有统计学意义(P <0.05);与有淋巴结转移的结肠腺癌组比较,无淋巴结转移的结肠腺癌组MGMT阳性表达升高,galectin-3阳性表达降低,差异有统计学意义(P <0.05);MGMT在结肠腺癌组织中的表达随着TNM分期增高而降低,galectin-3的表达随着TNM分期增高而增高,差异有统计学意义(P <0.05);MGMT和galectin-3的表达与结肠腺癌患者的年龄、性别无关(P> 0.05)。MGMT和galectin-3在结肠腺癌中的表达呈负相关(r=-0.399,P=0.002);MGMT和galectin-3在结肠腺癌中的阳性表达率与患者的3年生存率明显相关(P <0.05)。结论 MGMT和galectin-3在结肠腺癌的发生、发展过程中起重要作用,检测结肠腺癌组织中MGMT和galectin-3的表达有助于判断肿瘤的恶性程度、病情进展及预后。Objective To investigate the expression and clinical significances of DNA repair enzyme O6-methylguanine DNA methyltransferase(MGMT)and galectin-3in colon adenocarcinoma tissues collected with fibro colonoscopy.Methods From January2010to January2015,the tissues with fibro colonoscopy from120patients treated in Minzu Hospital of Guangxi Zhuang Autonomous Region were collected,including60colon adenocarcinomas,30inflammatory bowel diseases,and30colonic mucosal atypical hyperplasias.The expression of MGMT and galectin-3were measured by immunohistochemical SP method and the prognosis was evaluated by follow-up visit.Results Compared with the inflammatory bowel disease group,the positive expression of MGMT significantly decreased and the positive expression of galectin-3significantly increased in the colon adenocarcinomas group,the differences were statistically significant(P<0.05).Compared with medium-high differentiation colon adenocarcinoma group,the positive expression of MGMT significantly decreased and the positive expression of galectin-3significantly increased in poor differentiation colon adenocarcinoma group,the differences were statistically significant(P<0.05);the expression of MGMT in colon adenocarcinoma decreased with the increase of TNM stage,and the expression of galectin-3increased with the increase of TNM stage,the differences were statistically significant(P<0.05);the expression of MGMT and galectin-3were not related to age and gender(P>0.05).There was a negative association between the expression level of MGMT and galectin-3in colon adenocarcinoma(r=-0.399,P=0.002);the positive expression rate of MGMT and galectin-3in colon adenocarcinoma were significantly correlated with the3-year survival rate of patients(P<0.05).Conclusion MGMT and galectin-3might play important roles in tumorigenesis and development of colon adenocarcinoma,they are promising biomarkers for colon adenocarcinoma staging,metastasis and prognosis.

关 键 词：炎性肠黏膜病变 结肠黏膜不典型增生 结肠腺癌 O6-甲基鸟嘌呤-DNA甲基转移酶 半乳糖凝集素-3 

分 类 号：R574.62[医药卫生—消化系统]