生物标志物CD52对胰腺癌诊断及预后判断的作用:基于TCGA的数据分析  

The expression of CD52 in pancreatic cancer predicts poor prognosis:a study based on TCGA data

作　　者：赵力波 张蓉 ZHAO Libo;ZHANG Rong(Laboratory Medicine,Beijing Xuanwu Traditional Chinese Medical Hospital,Beijing 100050,China)

机构地区：[1]北京市宣武中医医院检验科,100050

出　　处：《国际检验医学杂志》2018年第A02期64-67,共4页International Journal of Laboratory Medicine

摘　　要：目的分析生物标志物CD52在胰腺癌组织中的表达差异及其对临床早期诊断,预后评估的作用,并结合基因富集分析探讨其潜在的作用机制,为后续研究提供线索。方法下载TCGA数据库中mRNA表达数据及胰腺癌患者相关临床病理参数,基于R语言进行数据的整理、合并及标准化;基于CD52基于表达差异对肿瘤样本分组,T检验统计CD52基因表达量在组间差异(P<0.05),随后Kaplan-Meier进行生存分析,最后结合GSEA分析调控CD52表达的潜在信号通路。结论肿瘤组织高表达CD52与患者TNM分期(Ⅱ期与Ⅲ期)及预后显著相关,原发性免疫缺陷反应、T细胞受体信号通路、NK细胞介导的细胞毒反应及Toll样受体等多个信号转导通路参与了CD52的表达调控。CD52或可成为判断胰腺癌预后,早期诊断及治疗恶性肿瘤的新靶点。Objective CD52 was previously reported to be an indicator of disease activity in chronic lymphocytic leukemia.In this study,we evaluated the roles of CD52 in pancreatic cancer using public available data from The Cancer Genome Atlas(TCGA).Methods The relationship between clinical pathologic features and the expression levels of CD52 were analyzed with student T-test.Clinicopathologic characteristics associated with overall survival in TCGA patients using the Kaplan-Meier method.Gene Set Enrichment Analysis(GSEA)was performed using TCGA data set.Conclusion Up-regulated CD52 expression was associated with TNM stage,especially with stage II and stage III.Furthermore,the univariate analysis revealed that CD52-high correlated significantly with a poor overall survival.GSEA show that primary immunodeficiency,T cell receptor signal pathway,NK cell mediated cytotoxicity and Toll like receptor signal pathway are differentially enriched in CD52 high expression phenotype.

关 键 词：胰腺癌 CD52 TCGA数据库 

分 类 号：R739.65[医药卫生—肿瘤]