脑卒中患者外周血CD4^+CD25^+及CD4^+CD28^-T细胞的变化及临床意义  

Changes and clinical significance of CD4^+CD25^+ and CD4^+CD28^-T cells in peripheral blood of patients with stroke.

作　　者：李定安[1] 陈思宇 李红妮 LI Ding-an;CHEN Si-yu;LI Hong-ni(No.1 Ward,Neurology Department,Hanzhong Central Hospital,Hanzhong 723000,Shaanxi,China;Neurology Department,the Fourth Military Medical University,Xi’an 71000,Shaanxi,China;Neurology Department,Xi’an TCM Brain Disease Hospital,Xi’an 710032,Shaanxi,China)

机构地区：[1]陕西省汉中市中心医院神经内科一病区,陕西汉中723000 [2]空军军医大学神经内科,陕西西安71000 [3]陕西省西安中医脑病医院神经内科,陕西西安710032

出　　处：《海南医学院学报》2018年第23期2082-2085,2089,共5页Journal of Hainan Medical University

基　　金：陕西省科学技术厅重点研发计划(2017SF-074)~~

摘　　要：目的:研究脑卒中患者外周血CD4^+CD25^+及CD4^+CD28^-T细胞的变化及其与神经元损害标志物、炎症细胞因子、斑块稳定性指标的相关性。方法:选择在我院诊断为急性缺血性脑卒中的患者作为研究的脑卒中组,另取同期体检的健康志愿者作为对照组。采集外周血并测定CD4^+CD25^+及CD4^+CD28^-T细胞的含量,采集血清并测定神经元损害标志物、炎症细胞因子、斑块稳定性指标的含量。结果:脑卒中组外周血中CD4^+CD25^+的含量以及血清中BDNF、IGF-1、IL-10、TGF-β1、TIMP2、Vaspin的含量低于对照组,外周血中CD4^+CD28^-T细胞的含量以及血清中NSE、VILIP-1、ET-1、IL-6、CX-CL12、VCAM-1、P-selectin、ox-LDL、CatS、ICTP、VEGF的含量的含量高于对照组;脑卒中组外周血中CD4^+CD25^+的含量与血清中BDNF、IGF-1、IL-10、TGF-β1、TIMP2、Vaspin的含量呈正相关,NSE、VILIP-1、ET-1、IL-6、CXCL12、VCAM-1、P-selectin、ox-LDL、CatS、ICTP、VEGF的含量呈负相关;外周血中CD4^+CD28^-T细胞的含量与血清中BDNF、IGF-1、IL-10、TGF-β1、TIMP2、Vaspin的含量呈负相关,NSE、VILIP-1、ET-1、IL-6、CXCL12、VCAM-1、P-selectin、ox-LDL、CatS、ICTP、VEGF的含量呈正相关。结论:脑卒中患者外周血CD4^+CD25^+及CD4^+CD28^-T细胞的变化能够加重神经元损害、促进炎症反应激活及斑块稳定性下降。Objective:To study the changes of CD4^+CD25^+and CD4^+CD28^-T cells in peripheral blood of patients with stroke and their correlation with neuronal damage markers,inflammatory cytokines and plaque stability indicators.Methods:The patients who were diagnosed with acute ischemic stroke in our hospital between June 2014 and December 2017 were selected as the stroke group of the research,and healthy volunteers who received physical examination during the same period were selected as the control group.Peripheral blood was collected to determine the contents of CD4^+CD25^+and CD4^+CD28^-T cells,and serum was collected to determine the contents of neuron damage markers,inflammatory cytokines and plaque stability indicators.Results:Peripheral blood CD4+CD25+cell content as well as serum BDNF,IGF-1,IL-10,TGF-β1,TIMP2 and Vaspin contents of stroke group was lower than those of control group(P<0.05)whereas peripheral blood CD4^+CD28^-T cell content as well as serum NSE,VILIP-1,ET-1,IL-6,CXCL12,VCAM-1,P-selectin,ox-LDL,CatS,ICTP and VEGF contents was higher than those of control group(P<0.05);peripheral blood CD4^+CD25^+cell content of stroke group was positively correlated with serum BDNF,IGF-1,IL-10,TGF-β1,TIMP2 and Vaspin contents,and negatively correlated with NSE,VILIP-1,ET-1,IL-6,CXCL12,VCAM-1,P-selectin,ox-LDL,CatS,ICTP and VEGF contents;peripheral blood CD4^+CD28^-T cell content was negatively correlated with serum BDNF,IGF-1,IL-10,TGF-β1,TIMP2 and Vaspin contents,and positively correlated with NSE,VILIP-1,ET-1,IL-6,CXCL12,VCAM-1,P-selectin,ox-LDL,CatS,ICTP and VEGF contents.Conclusion:The changes of CD4^+CD25^+and CD4^+CD28^-T cells in peripheral blood of patients with stroke can aggravate the neuron damage and promote the inflammatory response activation and plaque stability decline.

关 键 词：脑卒中 CD4^+CD25^+T细胞 CD4^+CD28^-T细胞 炎症反应 斑块性质 

分 类 号：R743.3[医药卫生—神经病学与精神病学]