高效液相色谱法同时测定甜油中苯甲酸、山梨酸、安赛蜜、糖精钠与脱氢乙酸  

Determination of Benzoic acid, Sorbic acid, Saccharin sodium, Acesulfame K, Dehydroacetic Acid in Sweet Oil by HPLC

作　　者：戚繁 王少伟 颜春荣 徐春祥 Qi Fan,Wang Shaowei,Yan Qi Fan;Wang Shaowei;Yan Chunrong;Xu Chunxiang(Jiangsu Institute of Food and Drug Supervision and Inspection,Nanjing 210000,China)

机构地区：[1]江苏省食品药品监督检验研究院,江苏南京210000

出　　处：《现代食品》2018年第20期101-104,共4页Modern Food

摘　　要：建立了甜油中苯甲酸、山梨酸、安赛蜜、糖精钠与脱氢乙酸5种食品添加剂的高效液相色谱法快速同时测定的方法。采用C_(18)色谱柱,以甲醇-0.02mol·L^(-1)乙酸钠与0.4%乙酸(25∶75)为流动相,流速1.0mL·min^(-1),柱温30℃,二极管阵列检测器,检测波长214nm、230nm、293nm。结果表明:该方法 5种添加剂回归方程线性关系良好,相对标准偏差在3.8%以下(n=6),回收率在93.7%～101.9%。该方法简便快速,准确可靠,可用于甜油中5种添加剂含量的同时测定。The analysis is performed on a C18 column using methanol and 0.02 mol·L-1sodium acetate solution and 0.04%acetic acid(25∶75)as the elution.The flow rate is 1.0 mL·min-1,the column temperature is 30℃.The detector is DAD,the detection wavelength are at 214 nm,230 nm and 293 nm.The results show that a good linear relationship is obtained.The RSD is below 3.8%(n=6),the average recovery is 93.7%~101.9%.The method is simple,rapid,repeatable and which could beapplied for the analysis of additives in sweet oil.

关 键 词：甜油 防腐剂 甜味剂 高效液相色谱法 

分 类 号：TS207[轻工技术与工程—食品科学]