高产纤维素酶菌株的筛选及产酶条件的研究  

The Research of Screening and Culture Conditions of a Hign Eellulose-producting Strain

作　　者：周新萍[1] Zhou Xinping(Department of City and Environmental Sciences, City College of Dongguan University of Technology, Dongguan 523419, China)

机构地区：[1]东莞理工学院城市学院城市与环境科学系,广东东莞523419

出　　处：《广东化工》2018年第24期13-14,共2页Guangdong Chemical Industry

基　　金：2014年市社会科技发展项目(高校科研一般项目201450715100106)

摘　　要：植物纤维是自然界最丰富的资源,筛选高产纤维素酶菌株,降低纤维素酶的生产成本,让植物纤维变废为宝一直是研究热点。从腐烂植物叶片中分离到一株高产纤维酶菌株CXYJ-1。对CXYJ-1菌株进行发酵培养条件的研究,结果表明,以豆饼粉为碳源,(NH_4)_2SO_4为氮源,培养温度为35～37℃,初始pH=5,培养96 h,产酶活力最高,CMC酶活为4.8 U/mL,FP酶活为4.5 IU/mL。Plant fiber is the most abundant resource in nature.Screening high-yield cellulase strains,reducing the cost of cellulase production,and turning plant fiber waste into treasure have always been a research hotspot.A high yield cellulase strain CXYJ-1was isolated from the leaves of decaying plants.The fermentation conditions of CXYJ-1strain were studied.The results showed that the activity of CMC and FP was4.8U/mL and4.5IU/mL with bean cake powder as carbon source,(NH4)2SO4as nitrogen source,culture temperature35-37℃,initial pH=5and96h.

关 键 词：高产 纤维素酶 产酶条件 

分 类 号：TQ[化学工程]