白芍提取物对经前期综合征肝气逆证模型大鼠海马中雌激素β受体及5-羟色胺转运体的影响  

Effects of Radices Paeoniae Alba extract on ERβand SERT in hippocampus of rat model of PMS liver-qi inverse syndrome

作　　者：王杰琼[1,2] 孙文君 田溪 李荟好 孙亚 王帅 郜彦鑫 WANG Jie-qiong;SUN Wen-jun;TIAN Xi;LI Hui-hao;SUN Ya;WANG Shuai;GAO Yan-xin(School of Pharmacy, Shandong University of Traditional Chinese Medicine, Ji'nan 250355,China;School of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Ji'nan 250355,China;Institute of Basic Theory of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Ji'nan 250355,China)

机构地区：[1]山东中医药大学药学院,山东济南250355 [2]山东中医药大学中医基础理论研究所,山东济南250355 [3]山东中医药大学中医学院,山东济南250355

出　　处：《中国药理学通报》2019年第1期46-51,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金青年基金资助项目(No 81603510)

摘　　要：目的探讨白芍提取物对PMS肝气逆证模型大鼠中枢海马ERβ及其介导的5-HT能系统中SERT的影响。方法筛选动情周期规律大鼠纳入实验,随机分为正常组、模型组、白芍提取物组、氟西汀组和ERβ激动剂组。采用电刺激复制PMS肝气逆证大鼠模型,给药5 d后,分离各组大鼠海马组织,采用RT-PCR法和免疫荧光组织化学法检测各组大鼠动情接受期和非接受期ERβ、SERT mRNA和蛋白表达。结果动情接受期的各组大鼠间旷场行为学得分,以及海马中ERβ、SERT mRNA和蛋白表达差异均无显著性。动情非接受期:与正常组相比,模型组大鼠旷场实验总路程明显增加;海马中ERβmRNA表达明显降低,SERT mRNA表达明显升高;模型组大鼠海马CA1、CA3脑区ERβ阳性细胞数和蛋白表达下降,SERT阳性细胞数和蛋白表达均明显上调;给予白芍提取物后,上述指标均得到明显改善。结论白芍提取物可能通过改善PMS肝气逆证大鼠海马区ERβ、SERT mRNA和蛋白的表达,发挥调节PMS肝气逆证负性情绪的作用。Aim To investigate the effect of Radices Paeoniae Alba extract on estrogen receptorβ(ERβ)and serotonin transporter(SERT)in the hippocampus of rat model of premenstrual syndrome(PMS)liver-qi inverse syndrome.Methods The rats were randomly divided into the normal group,the model group,the Radices Paeoniae Alba extract group,the fluoxetine group and the ERβagonist group by the vaginal smear microscopy and the open field score.The model group rats were prepared by electrical stimulation to prepare PMS liver-qi inverse syndrome.After five days of administration,the hippocampal tissues of each group were separated.ERβand SERT mRNA and protein expression in each group were determined by RT-PCR and immunofluorescence histochemical method.Results During the estrous reception period,there was no significant difference in the behavior score of the open-field test and the expression of ERβand SERT mRNA and protein in the hippocampus of each group.Estrous non-reception period:compared with normal rats,the total distance of open field test in model rats increased significantly.The expression of ERβmRNA in hippocampus significantly decreased and SERT mRNA significantly increased.In model group,the pyramidal cells in hippocampal CA1and CA3brain areas of rats were irregularly arranged,and the number of ERβpositive cells and protein expression decreased.The number of SERT positive cells and protein expression were significantly up-regulated.After the Radices Paeoniae Alba extract was given,the above indexes were significantly improved,and no significant difference was found between them and fluoxetine group and ER agitator group.Conclusions Radices Paeoniae Alba extract may adjust the PMS card of diseases with inverse negative emotions possibly by improving the hippocampus ERβand SERT mRNA and protein expression.

关 键 词：白芍提取物 经前期综合征肝气逆证 动情接受期和非接受期 海马 雌激素Β受体 5-羟色胺转运体 

分 类 号：R-332[医药卫生] R284.1