细胞块和免疫细胞学在浆膜腔积液检查中的临床病理分析  

Clinicopathological analysis of cell mass and immunocytology inserous cavity effusion

作　　者：刘科[1] 王雯[1] LIU Ke;WANG Wen(Department of Pathology Shangluo Central Hospital in Shaanxi Province,Shangluo 726000)

机构地区：[1]陕西省商洛市中心医院病理科,商洛726000

出　　处：《陕西医学杂志》2019年第1期114-116,共3页Shaanxi Medical Journal

摘　　要：目的:分析浆膜腔积液细胞块技术联合免疫细胞化学在浆膜腔积液细胞学病理诊断中的临床价值。方法:收集98例浆膜腔积液标本,进行常规细胞学涂片,细胞块切片HE染色,并用98例细胞块切片做甲状腺转录因子1(Thyroid transcription factor-1,TTF-1),人绒毛蛋白(Villin),癌胚抗原(CEA),钙视网膜蛋白(Calretinin),NapsinA、CDX-2、Pax-8行免疫细胞化学染色。结合临床一般资料和随访结果进行统计学分析。结果:98例浆膜腔积液中,常规细胞学涂片阳性率为25.5%,阴性率为64.3%,不确定率为10.2%;细胞块切片HE染色阳性率为26.5%,阴性率为62.2%,不确定率为11.3%;常规细胞学涂片和细胞块切片HE染色检查结果的差异无统计学意义(P>0.05)。细胞块切片免疫细胞化学染色阳性率为38.8%,阴性率为56.1%,不确定率为5.1%;综合诊断阳性率为38.8%,阴性率为57.14%,不确定率为4.08%;细胞块切片免疫细胞化学染色不确定率低于常规细胞学涂片和细胞块切片HE染色(P<0.05)。结论:细胞块切片免疫细胞化学染色与常规细胞学涂片相比,明显提高浆膜腔积液的阳性检出率,且对肿瘤细胞的来源判断有较大帮助。Objective:to analyze the clinical value of cell mass technique combined with immunocytochemistry in the pathological diagnosis of serous effusion.Methods:98 samples of serous effusion were collected for routine cytological smear and HE staining of cell sections.TIF-1,villin,CEA,Calretinin,NapsinA,CDX-2,Pax-8was stained by immunocytochemical staining.Statistics combined with general clinical data and follow-up results Scientific analysis.Results:the positive rate of routine cytologic smear was25.5% in 98 cases of serous cavity effusion,the negative rate was64.3%and the uncertainty rate was 10.2%.The positive rate of HE staining was 26.5%,the negative rate was 62.2% and the uncertainty rate was 11.3%.There was no significant difference in the results of HE staining between conventional cytologic smears and cell sections(P>0.05).The positive rate of immunocytochemical staining was38.8%,the negative rate was 56.1%,the uncertainty rate was 5.1%,the positive rate of comprehensive diagnosis was 38.8%,the negative rate was 57.14%.The fixed rate was 4.08 and the uncertainty rate of immunocytochemical staining was lower than that of conventional cytological smear and HE staining of cell slice(P<0.05).Conclusion:compared with conventional cytological smears,immunocytochemical staining of cell sections can significantly improve the positive detection rate of serous effusion,and can be of great help in judging the origin of tumor cells,which is worth popularizing.

关 键 词：浆膜腔积液 细胞块切片 免疫组织化学染色 病理分析 HE染色 细胞学涂片 

分 类 号：R73-3[医药卫生—肿瘤]