Reg Ⅳ mRNA在结直肠癌的表达及临床意义  

Expression and Clinical Significance of Reg Ⅳ mRNA in Colorectal Cancer

作　　者：常建兰[1] 于俊岩[1] 王晓燕[2] 李祖国[2] 郭红亮[1] 田向阳[1] 刘平[1] Chang Jianlan;Yu Junyan;Wang Xiaoyan(Department of Oncology,Affiliated Heping Hospital of Changzhi Medical College)

机构地区：[1]长治医学院附属和平医院肿瘤科,046000 [2]南方医科大学病理学系

出　　处：《长治医学院学报》2018年第6期401-403,407,共4页Journal of Changzhi Medical College

基　　金：国家自然科学基金(81800456);长治医学院博士启动基金(BS15008)

摘　　要：目的:分析RegⅣmRNA在新鲜结直肠癌患者的表达及其与性别、淋巴结转移、临床分期之间的关系。方法:收集长治医学院附属和平医院和南方医科大学附属南方医院结直肠癌患者新鲜结直肠癌组织30例和正常结直肠组织30例,采用实时荧光定量RT-PCR方法检测结直肠癌组织及正常组织中RegⅣmRNA表达水平。结果:RegⅣmRNA在结直肠癌组的表达量是正常结直肠组表达量的8.73倍(t=3.721,P<0.05),RegⅣmRNA在结直肠癌的表达和患者的性别无关(t=0.074,P>0.05),与淋巴结转移呈正相关(t=4.369,P<0.05),与临床分期无关(F=0.398,P>0.05)。结论:RegⅣmRNA在结直肠癌高表达,RegⅣmRNA的表达可能与结直肠癌患者的淋巴结转移密切相关。Objective:To analyze the expression of Reg Ⅳ mRNA in colorectal cancer patients and its relationship with gender,lymph node metastasis and clinical stage.Methods:30 cases of fresh colorectal cancer tissues and 30 cases of normal colorectum tissues were collected from patients in surgery department of Peace Hospital Affiliated to Changzhi Medical College and South Hospital of Southern Medical University in this study.Fresh tissues from patients’colorectal cancer and normal colorectum were divided into colorectal cancer group and normal group.The expression levels of Reg Ⅳ mRNA was detected by real time RT-PCR.Results:The expression of Reg Ⅳ mRNA in colorectal cancer group was 8.73 times higher than that of the normal group(t=3.721,P<0.05).Its expression in colorectal cancer group was positively correlated with lymphatic invasion(t=4.369,P<0.05)but not related to gender(t=0.074,P>0.05)or clinical stage(F=0.398,P>0.05).Conclusion:Reg Ⅳ mRNA is highly expressed in colorectal cancer,and the expression of Reg Ⅳ mRNA may be closely related to lymphatic metastasis of colorectal cancer patients.

关 键 词：REG Ⅳ 实时荧光定量RT-PCR 结直肠癌 淋巴结转移 

分 类 号：R735.3[医药卫生—肿瘤]