液相色谱-三重四极杆质谱联用法测定一次性使用输液器中偏苯三酸三辛酯的溶出量  

Determination of TOTM released from disposable infusion set by LC-MS/MS

作　　者：刘向辉 汤晓阳 孙小莉 刘东 

机构地区：[1]河南省医疗器械检验所,河南郑州450018

出　　处：《河南科技学院学报（自然科学版）》2018年第6期65-68,共4页Journal of Henan Institute of Science and Technology(Natural Science Edition)

基　　金：河南省科技计划(182102210501)

摘　　要：使用C8色谱柱,以体积分数为90%的甲醇水溶液为流动相,等度洗脱,多反应离子检测模式,监测离子对为m/z547.7→m/z305.4,建立了一次性使用输液器中偏苯三酸三辛酯(TOTM)溶出量的液相色谱-三重四极杆质谱联用(LC-MS/MS)检测方法.结果表明:TOTM质量浓度为5～150 ng/mL时,线性关系良好(r=0.999 4),检出限为0.5 ng/mL,相对标准偏差均小于2.27%,回收率在98.40%～100.22%之间.该方法操作简便、灵敏、准确、重现性好,适用于一次性使用输液器中TOTM的溶出量分析.The LC-MS/MS method was developed and validated for the determination of the TOTM released from disposable infusion set.A C8column was used with the mobile phase of methanol:water(V/V=90∶10)by isocratic elution.The analyte(m/z547.7→m/z305.4)was monitored under MRM mode.The conclusion indicated that the peak area is linear with its contents in the range of5~150ng/mL(r=0.9994),the detection limit is0.5ng/mL.the RSD are less than2.27%,the recovery ratio is in the range of98.40%~100.22%.This method is convenient,sensitive with good accuracy and precision,so that it can serve well in the quantitative analysis of TOTM released from disposable infusion set.

关 键 词：偏苯三酸三辛酯(TOTM) 液相色谱-三重四极杆质谱联用仪(LC-MS/MS) 一次性使用输液器 溶出 

分 类 号：O657.72[理学—分析化学]