真核翻译起始因子4G1在非小细胞肺癌中的表达及意义  

Expression of EIF4G1 in non-small cell lung cancer and its clinicopathological significance

作　　者：陆英[1] 虞珊珊[1] 马钻 白宝鑫 王光学[1] 徐增光[1] LU Ying;YU Shan-shan;MA Zuan;BAI Bao-xin;WANG Guang-xue;XU Zeng-guang(Center for Translational Medicine, East Hospital, Tongji University, Shanghai 200120, China)

机构地区：[1]同济大学附属东方医院转化医学研究中心,上海200120

出　　处：《同济大学学报（医学版）》2018年第6期60-65,共6页Journal of Tongji University(Medical Science)

基　　金：国家自然科学基金(81401303);上海市浦东新区优青人才培养计划(PWRq2015-08);上海市科学技术委员会科研计划(16411964800);上海市浦东新区科技发展基金(PKJ2015-Y16)

摘　　要：目的探讨真核翻译起始因子4G1(eukaryotic translation initiation factor 4 gamm 1,EIF4G1)在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达及意义。方法收集接受手术治疗的NSCLC患者81例(男性49例,女性32例),应用实时定量聚合酶链反应(PCR)和免疫组织化学方法检测癌组织及配对癌旁组织中EIF4G1mRNA和蛋白的表达,分析其在NSCLC癌组织中的表达特点及与患者临床病理特征的关系。结果 EIF4G1 mRNA在NSCLC癌组织中的表达(6. 92±1. 87)明显低于癌旁组织(8. 33±1. 69)(t=7. 01,P<0. 001)。将NSCLC分为3种不同病理类型:鳞癌、腺癌和其他类型,EIF4G1 mRNA在各组表达差异均具有统计学意义(P<0. 05)。EIF4G1mRNA在低分化癌组织中的表达水平(8. 90±1. 33)明显高于在中分化(1. 70±0. 17)和高分化(0. 84±0. 15)癌组织(F=14. 04,P<0. 001)。EIF4G1 mRNA在Ⅳ期癌组织中的表达(15. 42±3. 99)明显高于Ⅰ、Ⅱ、Ⅲ期癌组织(分别为1. 67±0. 37、2. 96±0. 77、5. 44±0. 99)(F=11. 84,P<0. 001)。不同年龄、性别和病理类型EIF4G1 mRNA表达水平均未见明显差异(均P>0. 05)。Logistic回归分析显示EIF4G1高表达与NSCLC癌细胞分化程度密切相关(OR=23. 74,P<0. 001)。免疫组化结果显示EIF4G1在低分化癌组织中的蛋白表达水平明显高于中分化和高分化癌组织(P<0. 05)。结论 EIF4G1在NSCLC中特异性高表达,且其表达强度与癌细胞的分化程度密切相关。Objective To investigate the expression of eukaryotic translation initiation factor4gamma1(EIF4G1)in non-small cell lung cancer(NSCLC)and its clinical significance.Methods The cancer tissues and paired adjacent normal lung tissues of81patients with NSCLC were collected.The mRNA and protein expression of EIF4G1was detected by qRT-PCR and immunohistochemistry,respectively.The relationship between EIF4G1expression and the clinicopathological features of NSCLC was analyzed.Results The ΔCt value of EIF4G1 mRNA in the cancer tissues was significantly lower than that in the matched adjacent tissues(6.92±1.87 vs.8.33±1.69,t=7.013,P<0.001).There were significant differences in EIF4G1 expression among different types of NSCLC(P<0.05).The expression level of EIF4G1 mRNA in poorly differentiated cancer tissues(8.90±1.33)was significantly higher than that in moderately differentiated(1.70±0.17)and highly differentiated(0.84±0.15)cancer tissues(F=14.04,P<0.001).The expression level of EIF4G1 mRNA in cancer tissues from the patients with clinical stage of Ⅳ(15.42±3.99)was significantly higher than that in stages I,II and III(1.67±0.37,2.96±0.77 and 5.44±0.99,F=11.84,P<0.001).There was no significant difference in the expression level of EIF4G1 mRNA between subjects with different age,sex and pathological types(P>0.05).Logistic regression analysis showed that high EIF4G1 expression was closely correlated with cell differentiation(OR=23.74,P<0.001).The immunohistochemical results showed that the protein expression level of EIF4G1in the low differentiated carcinoma tissues was significantly higher than that in the moderately and highly differentiated carcinoma tissues(P<0.05).Conclusion EIF4G1is highly expressed in NSCLC,and its expression level is closely related to the cell differentiation of NSCLC.

关 键 词：非小细胞肺癌 真核翻译起始因子4G1 实时定量聚合酶链反应 免疫组化 

分 类 号：R734.2[医药卫生—肿瘤]