真菌菌体直接PCR扩增的方法研究  

Direct PCR Amplification of Fungal Colony

作　　者：林艳云[1] 陈忆婷 黄晓明 林峻[2] Lin Yan-yun;Chen Yi-ting;Huang Xiao-ming;Lin Jun(Fuzhou University Zhicheng College,Fujian Fuzhou 350001;College of Biological Science and Engineering Fuzhou University,Fujian Fuzhou 350108)

机构地区：[1]福州大学至诚学院,福建福州350001 [2]福州大学生物科学与工程学院,福建福州350108

出　　处：《生物化工》2018年第6期7-10,共4页Biological Chemical Engineering

基　　金：国家自然科学基金项目(No.31301537)

摘　　要：真菌属于真核微生物,它种类繁多,与人类的健康密切相关,同时某些真菌还能被安全地利用于食品生产和生物化工领域。真菌分子生物学研究是微生物研究的一个重要领域,但由于真菌的细胞壁结构特殊,抗逆性强,使得真菌的核酸提取尤其困难。为实现对真菌基因组的快速提取与扩增,本研究尝试利用市售常见的细胞裂解液对4种典型真菌的细胞进行破壁处理,然后直接使用PCR技术实现对真菌基因组的快速扩增。结果显示黑曲霉513.88(Aspergillus niger 513.88)和里氏木霉(Trichoderma reesei)这两种重要的真菌可以使用细胞裂解液处理后实现直接PCR扩增。Fungi are a kind of eukaryotes,which are wide variety and closely related to human health.Some fungi can be safely used in food production and biochemical industries.The study of fungal molecular biology is an important field of microbial research,but the extraction of fungal nucleic acid is especially difficult because of the unique cell wall structure and strong resistance.In order to achieve rapid extraction and amplification of fungal genomes,this study attempted to use commercially available cell lysis solutions to break the walls of the cells of four fungi,and then PCR was performed later.The results showed that Aspergillus niger and Trichoderma reesei are suitable with this method.

关 键 词：真菌基因组 裂解 直接PCR扩增 

分 类 号：Q503[生物学—生物化学]