甲泼尼龙醋酸酯对重组白细胞介素-1β介导的颞下颌关节炎症损伤大鼠软骨细胞MMPs表达的影响  

Influence of methylprednisolone acetate on cartilage cells MMPs expression of rats with temporomandibular joint inflammatory injury mediated by recombinant interleukin-1β

作　　者：曹腾腾 叶蕾[1] 郑海霞 张引成[2] CAO Teng-teng;YE Lei;ZHENG Hai-xia(Department of Stomatology, The First Hospital of Yulin, Yulin Shaanxi 718000, China)

机构地区：[1]陕西省榆林市第一医院口腔科,陕西榆林718000 [2]西安交通大学口腔医院口腔颌面外科,陕西西安710004

出　　处：《临床和实验医学杂志》2019年第1期9-14,共6页Journal of Clinical and Experimental Medicine

摘　　要：目的探究甲泼尼龙醋酸酯对重组白细胞介素-1β(rh IL-1β)介导的颞下颌关节炎大鼠软骨细胞中基质金属蛋白酶1(MMP-1)、基质金属蛋白酶-3(MMP-3)、基质金属蛋白酶-9(MMP-9)表达的影响。方法以雄性SD大鼠作为研究对象,采用关节腔注射rh IL-1β法制备颞下颌关节炎大鼠模型,HE染色法观察颞下颌关节变化;对照组不进行任何处理。模型制备后将大鼠分为模型组、甲泼尼龙醋酸酯低剂量组(5 mg/kg)、中剂量组(15 mg/kg)、高剂量组(30 mg/kg),每组10只。对照组、模型组经腹腔静脉注射1 ml生理盐水,甲泼尼龙醋酸酯各剂量组分别经腹腔静脉注射甲泼尼龙醋酸酯。HE染色、番红染色观察各组颞下颌关节组织变化,采用Mankin's评分对颞下颌关节炎程度进行评定。RT-PCR法、Western Blot法、免疫组化法检测软骨组织中MMP-1、MMP-3、MMP-9的表达。结果 HE染色显示模型组大鼠颞下颌关节滑膜出现炎症细胞浸润、血管扩张、细胞黏附。与对照组相比,模型组Mankin's评分升高,与模型组相比,给药物组评分降低,且随着药物浓度的升高,评分逐渐下降(P <0. 05)。与对照组相比,模型组MMP-1、MMP-3、MMP-9 mRNA、蛋白表达量均升高,与模型组相比,给药组MMP-1、MMP-3、MMP-9 mRNA、蛋白表达量均降低,且随着药物浓度的升高,三者表达量均逐渐降低(P <0. 05)。免疫组化结果显示,模型组阳性细胞表达率高于对照组,经药物处理后MMP-1、MMP-3、MMP-9阳性细胞表达率随药物剂量的增加呈剂量性降低(P <0. 05)。结论甲泼尼龙醋酸酯能够缓解rh IL-1β介导的颞下颌关节炎,其作用机制可能与下调软骨细胞中MMP-1、MMP-3、MMP-9的表达有关。Objective To explore the influence of methylprednisolone acetate on cartilage cells MMPs expression of rats with temporomandibular joint inflammatory injury mediated by recombinant interleukin-1β(rhIL-1β).Methods SD male rats were used as the study objects,and the model of temporomandibular arthritis in rats was built by using joint cavity injection rhIL-1βmethod,and HE staining method was used to observe the changes of Nie and mandible,and after modeling,those were divided into model group,methylprednisolone acetate low dose treatment group(5 mg/kg),medium dose treatment group(15 mg/kg),and high dose treatment group(30 mg/kg).In the control group and the model group,1 ml of normal saline was intraperitoneally injected,and each dose of methylprednisolone acetate was intraperitoneally injected with methylprednisolone acetate.Changes of the mandibular joint tissues in different groups were observed by HE staining and crocus staining.Degree of temporomandibular arthritis was evaluated by using Mankin's score.MMP-1,MMP-3 and MMP-9 expression in cartilage tissue was detected by using RT-PCR,Western,Blot and immunohistochemistry.Results HE staining showed that inflammation,cell infiltration,vasodilatation and cell adhesion were observed in the synovium of TMJ rats of the model group.Compared with that in the control group,Mankin's score increased in the control group,and compared with that in the model group,the score decreased in the drug group,with the increase of drug concentration,the score decreased gradually(P<0.05).Compared with those in the control group,MMP-1,MMP-3,MMP-9 and mRNA protein expression in the model group increased,and compared with those in the model group,MMP-1,MMP-3,MMP-9,mRNA protein expression reduced in the drug group,with the increase of drug concentration,the three expression decreased(P<0.05).Immunohistochemical staining showed that the expression rate of positive cells in the model group was higher than that in the control group,and the expression rate of MMP-1,MMP-3 and MMP-9 positive

关 键 词：大鼠 颞下颌关节炎 甲泼尼龙醋酸酯 重组白细胞介素-1β 基质金属蛋白酶-1 基质金属蛋白酶-3 基质金属蛋白酶-9 

分 类 号：R782[医药卫生—口腔医学]