响应面法优化酶法辅助提取民族药地板藤总多糖工艺研究  

Optimization of Enzymatic Extraction Technology of Total Polysaccharides from Ficus tikoua Bur.by Response Surface Methodology

作　　者：张文平[1] 张晓平[2] 马大龙 马雅鸽[1] 侯安国[1] ZHANG Wenping;ZHANG Xiaoping;MA Dalong;MA Yage;HOU Anguo(Yunnan University of TCM,Kunming 650500,Yunnan,China;College of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,Shandong,China;Department of Pharmacy,The Affiliated Hospital of Qingdao University,Qingdao 26600,Shandong,China)

机构地区：[1]云南中医学院中药学院,云南昆明650500 [2]山东中医药大学药学院,山东济南250355 [3]青岛大学附属医院,山东青岛26600

出　　处：《辽宁中医杂志》2018年第11期2386-2389,2462,共5页Liaoning Journal of Traditional Chinese Medicine

基　　金：云南省科技强省计划项目(2014AE007);云南省教育厅科学研究基金项目(2016ZDX081)

摘　　要：目的:优化酶法辅助提取民族药地板藤总多糖的工艺条件。方法:在单因素试验基础上,采用Box-BehnkenDesign-响应面法,以酶用量、酶解时间、提取料液比为响应因子,以地板藤总多糖的提取率为响应值,采用3因素3水平的BBD设计进行试验,研究各因素及其交互作用对地板藤中总多糖提取率的影响。结果:响应面分析法优化的最佳提取工艺为酶用量为1.5%、酶解时间63.31min、提取料液比1:32.92,预测最高提取率9.23%;验证试验中地板藤总多糖的提取率为8.51%(RSD=0.93%,n=5)。结论:酶法辅助提取地板藤总多糖的提取率较高,工艺合理、简便,可用作地板藤多糖的提取。Objective:To optimize the enzymatic extraction of total polysaccharides from Ficus tikoua Bur.Methods:The process was optimized by Box-behnken-response surface methodology based on the single factor tests.By using enzyme dosage,enzyme time and feed liquid ratio as response factors,total polysaccharides used as response value,3-factors and 3-level analysis was adopted for the experiment.Results:The optimal extraction technology was as enzyme dosage of 1.5%,enzyme time of 63.31 min and feed liquid ratio of 1:32.92,and the predicted highest extraction yield was 9.23 mg/g.In validation test,the extraction rate of total polysaccharides was 8.51%(RSD=0.93%,n=5).Conclusion:The optimized extraction technology is effective,reasonable and simple,and can be used for the extraction of total polysaccharides from Ficus tikoua Bur.

关 键 词：地板藤 响应面法 酶法提取 总多糖 

分 类 号：R284[医药卫生—中药学]