铜离子对锦鲤DNA的损伤  

DNA Damage of Cu^(2+) to Cyprinus carpio

作　　者：刘秋妤 陈新[1,2] 覃宗敏 卓文 唐敏[1,2] LIU Qiuyu;CHEN Xin;QIN Zongmin;ZHUO Wen;TANG Min(College of Materials and Chemical Engineering,Hainan University,Haikou,Hainan 570228,China;State Key Laboratory of Marine Resource Utilization in South China Sea (Hainan University),Haikou,Hainan 570228,China)

机构地区：[1]海南大学材料与化工学院,海口570228 [2]南海海洋资源利用国家重点实验室(海南大学),海口570228

出　　处：《热带生物学报》2018年第4期388-392,共5页Journal of Tropical Biology

基　　金：国家自然科学基金项目(31360105;31660128);海南省研究生创新科研课题(Hys2018-68;Hys2018-67)

摘　　要：通过微核和彗星试验检测了铜离子胁迫下锦鲤(Cyprinus carpio)外周血细胞DNA损伤情况。实验发现不同浓度铜离子胁迫48 h后,锦鲤外周血细胞的彗星率(17. 7%,44. 4%,60. 0%,88. 9%)和微核率(0. 4‰,8. 4‰,9. 8‰,12. 2‰)与铜离子浓度呈正相关。细胞尾部DNA含量(2. 91%,5. 74%,8. 43%,11. 28%)、尾长(47,74,108,112μm)和Olive尾距(4. 51,9. 39,17. 68,18. 62)也都随铜离子浓度升高而呈增长趋势,而且G2,G3组均显著高于对照组(P <0. 05)。2种试验方法所获结果相互印证,从不同角度揭示了铜离子对锦鲤外周血细胞的遗传毒性效应。特别是微核试验灵敏度较高,步骤简捷,成本低廉,在生态遗传毒性检测应用推广方面具有较大优势。Common carp(Cyprinus carpio)was cultured and exposed to different concentrations of Cu^2+,and the DNA damage of the peripheral blood cells of C.carpio was analyzed by using the micronucleus test and the comet assay.The results showed that the rates of comet(17.7%,44.4%,60.0%,88.9%)and micronucleus(0.4‰,8.4‰,9.8‰,12.2‰)of the peripheral blood cells of C.carpio in the control,G1,G2 and G3 were positively correlated with the concentration of Cu^2+after C.carpio were exposed to Cu^2+for 48 hrs.Under the stress of Cu^2+for 48h the tail DNA content(2.91%,5.74%,8.43%,11.28%),tail length(47,74,108,112μm)and Olive tail moment(4.51,9.39,17.68,18.62)in the control,G1,G2 and G3 all tended to increase with the rise of Cu^2+concentration and they were significantly higher in the groups G2 and G3 than in the control group(P<0.05).The results of either the micronucleus test or the comet assay corroborated the genetic damage by Cu^2+to the peripheral blood cells of C.carpio from different aspects.In particular,the micronucleus test was high in sensitivity,low in cost,and simple in steps and will have a high potential of application and extension in the test of ecogenotoxicity.

关 键 词：锦鲤 铜胁迫 DNA损伤 

分 类 号：X174[环境科学与工程—环境科学]