乙型病毒性肝炎、原发性肝细胞癌患者血清代谢组学研究  

Metabonomic studies on the serum of the patients with viral hepatitis B and Hepatocellular carcinoma

作　　者：杨文涛[1] 谢宝刚[2] 徐颜美[3] 刘亚兰[2] 邹书兵[4] YANG Wentao;XIE Baogang;XU Yanmei;LIU Yalan;ZOU Shubing(Department of Organ Transplant,the Second Affiliated Hospital of Nanchang University,Nanchang,Jiangxi 330006,China;College of Pharmacy,Nanchang University,Nanchang,Jiangxi 330006,China;Department of Clinical Laboratory,the Second Affiliated Hospital of Nanchang University,Nanchang,Jiangxi 330006,China;Department of Hepatobiliary Surgery,the Second Affiliated Hospital of Nanchang University,Nanchang,Jiangxi 330006,China)

机构地区：[1]南昌大学第二附属医院器官移植科,江西南昌330006 [2]南昌大学药学院,江西南昌33006 [3]南昌大学第二附属医院检验科,江西南昌330006 [4]南昌大学第二附属医院肝胆外科,江西南昌330006

出　　处：《国际检验医学杂志》2019年第1期29-33,共5页International Journal of Laboratory Medicine

基　　金：江西省科技支撑计划项目(20151BBG70158);江西省教育厅项目(GJJ150277)

摘　　要：目的研究乙型病毒性肝炎、原发性肝细胞癌(HCC)患者及健康体检者代谢物变化。方法收集49例HCC、29例乙型肝炎DNA阴性、26例乙型肝炎DNA阳性患者血清作为病例组,收集46例健康体检者血清作为对照组,采用AvanceⅡ-600MHz核磁共振仪检测血清中小分子代谢产物,通过聚类分析筛选出各组的特征代谢物。结果与对照组比较,乙型肝炎DNA阴性组患者血清中有12种代谢物质差异有统计学意义(P<0.05),乙型肝炎DNA阳性组患者血清中有15种代谢物质差异有统计学意义(P<0.05);肝癌组患者血清中有21中代谢物质差异有统计学意义(P<0.05),其中苯乙尿酸水平上升,其他代谢物下降。与DNA阳性组比较,乙型肝炎DNA阴性组患者血清中有14种代谢物质差异有统计学意义(P<0.05)。结论核磁共振氢谱可有效筛查出2型肝炎、HCC患者之间有显著差异的血清代谢物,在无创诊断HCC方面具有广阔的应用前景。Objective To explore the metabolite composition of the serum samples from the hepatitis,Hepatocellular carcinoma(HCC)patients and healthy controls.Methods The serum samples from 49 patients with HCC,29 hepatitis B patients with negative DNA,26 hepatitis B patients with positive DNA were collected as case group,and 46 healthy as control group.The small molecular metabolites were detected AvanceⅡ-600MHz,and the characteristic metabolites were screened by cluster analysis.Results Compared with the control group,there were 12 metabolites have the significant differences in the DNA negative hepatitis group,and 15 metabolites in the DNA positive hepatitis group,21 metabolites in the HCC group.Among the metabolites with significant differences in HCC,phenyletic acid was increased and other metabolites were decreased.Fourteen metabolites have the significant differences between the DNA negative hepatitis group with DNA positive hepatitis group.Conclusion Nuclear magnetic resonance hydrogen spectroscopy(NMR)can effectively screen the serum metabolites with significant differences between hepatitis B and HCC patients,and has broad prospects in the further non-invasive diagnosis of HCC.

关 键 词：磁共振波谱学 磁共振成像 癌 肝细胞 肝炎 乙型 代谢组学 

分 类 号：R735.7[医药卫生—肿瘤] R445.2[医药卫生—临床医学]