miRNA-21和miRNA-101表达水平对结直肠癌诊断价值的meta分析  

Meta-analysis of the diagnostic value of miRNA-21 and miRNA-101 expression levels in colorectal cancer

作　　者：储怀祝 韩娜慧 吕海栋[1] 曹洪涛[1] 徐建国[1] CHU Huaizhu;HAN Nahui;LU Haidong;CAO Hongtao;XU Jianguo(Department of Oncology Surgery,Qinghai People's Hospital,Xining,Qinghai 810007,China;Department of Pain,Qinghai People's Hospital,Xining,Qinghai 810007,China)

机构地区：[1]青海省人民医院肿瘤外科,青海西宁810007 [2]青海省人民医院疼痛科,青海西宁810007

出　　处：《国际检验医学杂志》2019年第1期80-84,88,共6页International Journal of Laboratory Medicine

摘　　要：目的探讨微小RNA-21(miRNA-21)和miRNA-101的表达水平对结直肠癌的诊断价值。方法检索PubMed、EMBASE、Cochranelibrary、维普资讯中文期刊服务平台(VIP)、中文生物医学期刊文献数据库(CMCC)、中国知网(CNKI)等数据库,收集2008-2018年采用miRNA-21和miRNA-101表达水平诊断结直肠癌的相关文献;采用Revman5.3和Meta-Disc1.4软件对数据进行meta分析,通过绘制森林图及受试者工作特征曲线(SROC)等统计指标综合评价miRNA-21和miRNA-101表达水平对结直肠癌的诊断价值。结果共收集文献534篇,纳入文献11篇,样本量为1 040例,其中病例组600例,对照组440例。纳入研究有异质性,采用随机效应模型,miRNA-21和miRNA-101表达水平诊断结直肠癌汇总灵敏度为0.77,汇总特异度为0.73,阳性似然比为2.97[95%可信区间(95%CI):2.13～4.13],阴性似然比为0.23(95%CI:0.10～0.56),诊断比值比为14.05(95%CI:6.39～30.91),SROC曲线下面积(AUC)为0.853 4。miRNA-21表达水平诊断结直肠癌汇总灵敏度为0.64,汇总特异度为0.75,阳性似然比为2.97(95%CI:1.95～4.54),阴性似然比为0.26(95%CI:0.09～0.78),诊断比值比为12.77(95%CI:4.60～35.43),AUC为0.855 9;miRNA-101表达水平诊断结直肠癌汇总灵敏度为0.88,汇总特异度为0.69,阳性似然比为2.80(95%CI:1.74～4.50),阴性似然比为0.20(95%CI:0.11～0.36),诊断比值比为15.56(95%CI:5.39～44.93),AUC为0.8791。结论 miRNA-21和miRNA-101表达水平对结直肠癌的诊断均具有一定的参考价值。Objective To investigate the diagnostic value of microRNA-21(miRNA-21)and miRNA-101 expression levels in colorectal cancer.Methods The databases of PubMed,EMBASE,Cochranelibrary,VIP Chinese Journal Service Platform(VIP),Chinese Biomedical Journal Literature Database(CMCC),China Knowledge Network(CNKI)were searched,and miRNA-21 and miRNA-101 were collected in 2008-2018.Expression level diagnosis of colorectal cancer;Meta-analysis of data using Revman 5.3 and Meta-Disc 1.4 software,comprehensive evaluation of miRNA-21 by statistical indicators such as forest map and receiver operating characteristic curve(SROC curve)And the diagnostic value of miRNA-101 expression levels for colorectal cancer.Results A total of 534 articles were collected and 11 articles were included in the literature.The sample size was 1 040 cases,including 600 cases in the case group and 440 cases in the control group.The inclusion studies were heterogeneous.The randomized effect model,miRNA-21 and miRNA-101 expression levels were used to diagnose colorectal cancer with a combined sensitivity of 0.77,a pooled specificity of 0.73,and a positive likelihood ratio of 2.97[95%confidence interval(95%CI):2.13 to 4.13],the negative likelihood ratio was 0.23(95%CI:0.10-0.56),the diagnostic odds ratio was 14.05(95%CI:6.39-30.91),and the area under the SROC curve(AUC)was 0.853 4.The sensitivity of miRNA-21 expression level for diagnosis of colorectal cancer was 0.64,the combined specificity was 0.75,the positive likelihood ratio was 2.97(95%CI:1.95-5.54),and the negative likelihood ratio was 0.26(95%CI:0.09-0.78).),the diagnostic odds ratio was 12.77(95%CI:4.60-35.43),AUC was 0.855 9;the sensitivity of miRNA-101 expression level for colorectal cancer was 0.88,the combined specificity was 0.69,and the positive likelihood ratio was 2.80(95%CI:1.74-4.50),the negative likelihood ratio was 0.20(95%CI:0.11-0.36),the diagnostic odds ratio was 15.56(95%CI:5.39-44.93),and the AUC was 0.8791.Conclusion The expression levels of miRNA-21 and miRNA-101 have certain re

关 键 词：结直肠肿瘤/诊断 微RNAS META分析 基因表达 

分 类 号：R735.34[医药卫生—肿瘤] Q786[医药卫生—临床医学]