SHR-SP脑梗死后微血管形成与星形胶质细胞的关系  

Relationship between microangiogenesis and astrocytes after cerebral infarct in SHR-SP rats

作　　者：侯惠莲[1] 田美丽[2] 张冠军[1] 王鸿雁[1] 宫惠琳[1] 李晓锋[1] 张学斌[1] HOU Hui-lian;TIAN Mei-li;ZHANG Guan-jun;WANG Hong-yan;GONG Hui-lin;LI Xiao-feng;ZHANG Xue-bin(Department of Pathology,The First Affiliated Hospital of Xi'an Jiaotong University Health Science Center,Xi'an 710061,China;Department of Oncology Radiotherapy,The First Affiliated Hospital of Xi'an Jiaotong University Health Science Center,Xi'an 710061,China)

机构地区：[1]西安交通大学第一附属医院病理科,陕西西安710061 [2]西安交通大学第一附属医院肿瘤放疗科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2019年第1期76-81,共6页Journal of Xi’an Jiaotong University（Medical Sciences）

摘　　要：目的探讨自发性高血压大鼠(stroke-prone,spontaneously hypertensive,SHR-SP)脑梗死周围半暗区微血管形成及其构筑变化与星形胶质细胞的关系。方法建立SHR-SP脑梗死模型,从SHR-SP大鼠股静脉注入FITCdextran后在共聚焦显微镜下观察大鼠脑血管的构筑,测定微血管直径及分支数;通过免疫组织化学及免疫荧光染色法检测SHR-SP脑梗死周围半暗区GFAP、FⅧ的表达,测定其GFAP阳性细胞数、微血管密度(microvessel density,MVD),分析SHR-SP脑梗死周围半暗区星形胶质细胞数量与MVD、微血管直径及分支数的相互关系。结果 SHRSP在饮用高盐(13g/L NaCl)水溶液后第7周开始出现脑梗死;与对照组WKY比较,在SHR-SP脑梗死周围半暗区GFAP表达的阳性细胞数、MVD、微血管分支数显著升高,微血管直径显著增粗,且明显高于对侧半球相应区及非高盐饮水SHR-SP脑的相应区(P<0.01)。直线相关分析显示,GFAP表达的阳性细胞数与MVD及微血管直径及分支数呈正相关性(P<0.01)。结论高盐(13g/L NaCl)饮水可诱发SHR-SP发生脑梗死;在SHR-SP脑梗死周围半暗区,微血管形成及构筑的变化促使了星形胶质细胞的增生。Objective To investigate the possible role of astrocytes after brain infarction in stroke-prone,spontaneously hypertensive(SHR-SP)rats and the association with angiogenesis and the architecture.Methods We maintained SHR-SP rats on high sodium water starting to accelerate the stroke onset.The 3D quantification of microvasculatures(diameter,branch number)by cofocal microscope after FITC-dextran was injected into the rats via the left femoral vein.Glial fibrillary acidic protein(GFAP)expression and microvessel density(MVD)using counting the number of factorⅧ-positive endothelial cells were evaluated by immunofluorescence and immunohistochemistry,respectively.Results Cerebral infarction occurred at week 7 after high sodium water intake(13 g/L NaCl)in SHR-SP group.When compared with the non-infarcted contralateral hemisphere and SHR-SP on normal sodium intake and WKY rats,GFAP expression and MVD were significantly increased,respectively,and the diameter and the branch number of vessels were decreased,respectively,in cerebral infarcts with boundary zones of SHR-SP rats(P<0.01).Linear correlation analysis showed that GFAP expression was positively correlated with MVD and the diameter and the branch number of vessels in cerebral infarcts in SHR-SP(P<0.01).Conclusion Astrocytes hyperplasia may be associated with increased regional angiogenesis and the changes of architecture in SHR-SP rats with high sodium water(13 g/L NaCl)that induces focal cerebral infarcts.

关 键 词：SHR-SP脑梗死 星形胶质细胞 胶质纤维酸性蛋白 微血管密度 微血管构筑 

分 类 号：R743.3[医药卫生—神经病学与精神病学]