siRNA下调AEG-1基因表达对胶质瘤细胞血管拟态形成的影响  

Effects of down-regulation of astrocyte elevated gene-1 expression by siRNA on glioma vasculogenic mimicy

作　　者：梁晨 唐荣华[1] 上官健[1] 廉民学[1] 郭世文[1] LIANG Chen;TANG Rong-hua;SHANGGUAN Jian;LIAN Min-xue;GUO Shi-wen(Department of Neurosurgery,The First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710061,China)

机构地区：[1]西安交通大学第一附属医院神经外科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2019年第1期87-91,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：陕西省自然科学基础研究计划资助项目(No.2017JQ8037)~~

摘　　要：目的探讨下调星型细胞上调基因-1(AEG-1)表达对胶质瘤细胞血管拟态(VM)形成的影响及其可能的机制。方法利用siRNA下调U87胶质瘤细胞中AEG-1表达,通过体外构建胶质瘤VM模型,研究下调AEG-1表达对胶质瘤VM形成的影响;Real-time PCR及Western blot分别检测AEG-1表达下调对U87细胞中血管内皮生长因子(VEGF)、基质金属蛋白酶-2(MMP2)mRNA及蛋白表达的影响;免疫荧光法检测AEG-1表达下调对VM模型中VEGF及MMP2表达的影响。结果 siRNA下调AEG-1表达能显著降低U87细胞体外VM的形成(P<0.01),同时下调AEG-1基因能够显著抑制U87细胞及其构成的VM中VEGF及MMP2的表达(P<0.01)。结论 siRNA下调AEG-1表达能显著抑制胶质瘤细胞VM的形成,其部分机制可能是通过下调VEGF及MMP2表达实现。Objective To investigate the effects of down-regulation of astrocyte elevated gene-1(AEG-1)expression by siRNA on glioma vasculogenic mimicy in vitro.Methods The expression of AEG-1 in U87 glioma cells was down-regulated by siRNA.An in vitro model of glioma vasculogenic mimicy was established to evaluate the effect of down-regulation of AEG-1 expression on glioma vasculogenic mimicy.Real-time PCR and Western blot were used to detect the mRNA and protein expressions of VEGF and MMP2 in U87 cells after treated with AEG-1 siRNA,respectively.Immunofluorescence was used to detect the expressions of VEGF and MMP2 in glioma vasculogenic mimicy model in vitro.Results Down-regulation of AEG-1 expression by siRNA could significantly inhibit the glioma vasculogenic mimicy in vitro(P<0.01).Meanwhile,It could also inhibit the expressions of VEGF and MMP2 in glioma cells and glioma vasculogenic mimicy in vitro(P<0.01).Conclusion These results suggest that down-regulation of AEG-1 expression by siRNA can significantly inhibit the glioma vasculogenic mimicy in vitro,and these effects may be realized partly through regulation of VEGF and MMP2 expression.

关 键 词：星型细胞上调基因-1 SIRNA 胶质瘤 血管拟态 

分 类 号：R739.4[医药卫生—肿瘤]