肝细胞癌癌组织和癌旁肝组织Ep-CAM和C-Kit表达及其意义探讨  

Cancerous and noncancerous expression of Ep-CAM and C-Kit in patients with hepatocellular carcinoma

作　　者：陈永其 陈前 袁春艳 刘军[1] 谢蕴 Chen Yongqi;Chen Qian;Yuan Chunyan(Department of Pathology,Central Hospital of Minhang District,Affiliated to Zhongshan Hospital,Fudan University,Shanghai 201199,China)

机构地区：[1]复旦大学附属中山医院闵行分院/上海市闵行区中心医院病理科,上海市201199 [2]复旦大学附属中山医院闵行分院/上海市闵行区中心医院普外科,上海市201199

出　　处：《实用肝脏病杂志》2019年第1期109-112,共4页Journal of Practical Hepatology

基　　金：复旦大学附属中山医院闵行医院院长基金资助项目(编号:2017MHLC01)

摘　　要：目的探讨肝细胞癌(HCC)患者癌组织和癌旁肝组织C-Kit蛋白和肿瘤干细胞标志上皮细胞黏附分子(EpCAM)蛋白表达的变化。方法 2014年3月～2017年1月我院经手术切除治疗的HCC患者癌组织和癌旁肝组织标本90份,采用免疫组化染色法检测癌组织和癌旁肝组织Ep-CAM蛋白和C-Kit蛋白表达情况,并比较不同分化、有无包膜、不同病灶大小、术前不同血清甲胎蛋白(AFP)水平癌组织Ep-CAM蛋白和C-Kit蛋白表达阳性率的差异。结果在本组90例HCC患者肝组织中,癌组织Ep-CAM蛋白和C-Kit蛋白表达阳性率分别为65.6%和74.4%,均显著高于癌旁组织的11.1%和4.4%,差异具有统计学意义(P<0.05);50例Ⅲ级和Ⅳ级组织学分化、49例术前血清AFP>400 ng/ml、28例发生血管浸润的癌组织Ep-CAM蛋白表达阳性率分别为76.0%、79.6%和82.1%,显著高于40例Ⅰ级和Ⅱ级组织学分化、41例血清AFP≤400 ng/ml和62例未发生肿瘤血管浸润的癌组织(分别为52.5%、48.8%和58.1%);Ⅲ级和Ⅳ级组织学分化、术前血清AFP>400 ng/ml、发生血管浸润的癌组织C-Kit蛋白表达阳性率分别为86.0%、87.8%和89.3%,也显著高于Ⅰ级和Ⅱ级组织学分化、血清AFP≤400 ng/ml和未发生肿瘤血管浸润的癌组织(分别为60.0%、58.5%和67.7%),差异均具有统计学意义(P<0.05)。结论 HCC患者癌组织Ep-CAM蛋白和C-Kit蛋白表达阳性率显著增高,并与肿瘤组织学分级、术前血清AFP水平和是否发生肿瘤血管浸润有关,其临床意义还有待进一步探讨。Objective To investigate the cancerous and noncancerous expression of epithelial cell adhesion molecule(Ep-CAM) and C-Kit in patients with hepatocellular carcinoma(HCC). Methods Cancerous and its adjacent noncancerous tissues from ninety patients with HCC were obtained after hepatectomy in our hospital between March 2015 and January 2017. Immunohistochemical staining was applied to detect the expression of EpCAM and C-Kit protein,and the positivities of Ep-CAM and C-Kit protein between different histological grades,with or without envelope,different lesion sizes,high and low serum alpha-fetoprotein(AFP) levels and with or without vascular infiltration were compared. Results Out of the 90 patients with HCC,the positive expression rates of Ep-CAM and C-Kit protein in cancerou tissues were 65.6% and 74.4%,significantly higher than 11.1%and 4.4% in its adjacent noncancerous tissues(P<0.05);the positive expression rates of Ep-CAM in 50 patients with Ⅲ/Ⅳ differentiation,in 49 with serum AFP >400 ng/ml and in 28 with vascular infiltration were 76.0%,79.6% and 82.1%,significantly higher than 52.5%,48.8% and 58.1% in 40 patients with Ⅰ/Ⅱ differentiation,in41 with serum AFP≤400 ng/ml and in 62 without vascular tumor infiltration;the positive expression rates of CKit in patients with Ⅲ/Ⅳ differentiation,in with serum AFP >400 ng/ml and in with vascular infiltration were 86.0%,87.8% and 89.3%,much higher than 60.0%,58.5% and 67.7% in patients withⅠ/Ⅱ differentiation,in with serum AFP ≤400 ng/ml and in without vascular tumor infiltration(P<0.05). Conclusion The positive expression rate of Ep-CAM protein and C-Kit protein in cancerous tissues of patients with HCC increase,and they are related to tumor histological grades,preoperative serum AFP levels,and to vascular tumor infiltration. The implication needs further investigations.

关 键 词：肝细胞癌 C-KIT蛋白 上皮细胞黏附分子 免疫组化 

分 类 号：R735.7[医药卫生—肿瘤]