核桃粕蛋白提取工艺优化及其酶解产物活性研究  

Optimization of extraction process of walnut meal protein and activity of enzymatic hydrolysate

作　　者：薛南南 房康 张玥辉 陈萌 王辉[1] 张欣钰 吴高荣 郭文博[1] 贾晓慧[1] 王鹏龙[1] 雷海民[1] XUE Nannan;FANG Kang;ZHANG Yuehui;CHEN Meng;WANG Hui;ZHANG Xinyu;WU Gaorong;Guo Wenbo;JIA Xiaohui;WANG Penglong;LEI Haimin(School of Chinese Pharmacy,Beijing University of Chinese Medicine,Beijing 102488,China)

机构地区：[1]北京中医药大学中药学院,北京102488

出　　处：《天津中医药》2019年第1期87-92,共6页Tianjin Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81603256;81173519);北京中医药大学创新团队资助项目(2011-CXTD-15);基于中药配伍的"酸碱络合"与"结构拼合"先导化合物发现研究(北京中医药大学;1000061222485);北京市重点实验室中药基础与新药研发(100102);中央高校基本科研业务费专项资金资助(BUCM-2019-JCRC002)

摘　　要：[目的]利用响应面法优化核桃粕蛋白提取工艺,并评价其木瓜蛋白酶解物的体外抗氧化活性,为核桃粕后续开发利用提供参考价值。[方法]采用碱溶酸沉法,以蛋白提取率为响应值,结合单因素及响应面实验探讨氢氧化钠(NaOH)浓度、液料比、提取温度及时间对核桃粕蛋白提取率的影响,优化核桃粕蛋白提取工艺,并利用体外抗氧化实验评价了木瓜蛋白酶解物的活性。[结果]响应面法优化的核桃粕蛋白提取工艺为:80∶1的0.04%NaOH溶液、65℃下提取1 h,平行实验的结果为36.01%,与预测值35.27%较接近,说明该工艺真实可靠、简单可行。核桃蛋白木瓜蛋白酶解物具有一定的清除DPPH、OH自由基及还原能力,可为后续的核桃粕保健食品开发与工业化生产提供方法及参考意义。[结论]优化了一种核桃粕蛋白提取工艺,并为核桃粕的综合开发及利用提供了重要的参考价值。[Objective] Optimize the extraction process of walnut meal protein by response surface methodology(RSM),and evaluate its antioxidant activity of papain hydrolysate,which provided a reference for the subsequent development and utilization of walnut meal protein.[Methods] The alkali-soluble acid precipitation method was used to optimize the parameters (NaOH concentration,liquid-to-material ratio,extraction temperature and time) of extraction process with the result of single factor and RSM experimentation.The papain hydrolysate was tested for its antioxidant activity.[Results] The result of RSM was as follows: 80∶1 liquid-to-material ratio,0.04% NaOH solution,65℃ for 1.0 h,with 35.27% of extraction rate.The extraction rate of three parallel experiments was up to 36.01% closed to the theoretical value,indicating that the process is real and reliable,convenient and feasible; papain hydrolysate of walnut protein effected on scavenging DPPH,OH radical and reducing activity,which could provide basis for the subsequent development and industrial production of walnut health food.[Conclusion] This paper successfully optimized the extraction process of walnut meal protein by RSM,and provided an important reference value for the comprehensive development and utilization of walnut meals.

关 键 词：核桃粕蛋白 提取工艺 优化 酶解产物 活性 

分 类 号：R283[医药卫生—中药学]