miR-30b在膀胱癌中表达的临床意义及对膀胱癌细胞生物学特性影响研究  

The clinical significance of miR-30b expression in bladder cancer and its effect on biological characteristics of bladder cancer cells

作　　者：李利军[1] 邱明星[1] 马志伟[1] 龚百生[1] LI Li-jun;QIU Ming-xing;MA Zhi-wei;GONG Bai-sheng(Department of Urology,Sichuan Academy of Medical Sciences & Sichuan Provincial People s Hospital,Chengdu 610072,China)

机构地区：[1]四川省医学科学院.四川省人民医院泌尿外科,四川成都610072

出　　处：《实用医院临床杂志》2019年第1期1-4,共4页Practical Journal of Clinical Medicine

摘　　要：目的探讨miR-30b在膀胱癌组织细胞中的表达及临床意义,并研究其对膀胱癌T24细胞生物学特性的影响。方法采用实时定量PCR检测32例膀胱癌及癌旁非肿瘤组织中miR-30b的表达水平,采用外源性转染使miR-30b水平上调,研究miR-30b水平上调对膀胱癌T24细胞的增殖、侵袭、凋亡等生物学特性的影响。结果肿瘤组织中miR-30b的表达水平低于癌旁组织(P <0. 05),临床分期与miR-30b的表达水平差异无统计学意义(P> 0. 05);转染miR-30b的T24细胞在48 h内的存活率及侵袭数均低于阴性对照组(P <0. 05)。结论 miR-30b水平与膀胱癌的发生发展可能有关,miR-30b导入膀胱癌细胞后,能够显著抑制癌细胞的侵袭能力,加速癌细胞的凋亡。Objective To investigate the expression and clinical significance of miR-30 b in bladder cancer cells and its effect on the biological characteristics of bladder cancer T24 cells.Methods Real-time quantitative PCR was used to detect the expression of miR-30 b in cancer and adjacent non-tumor tissues of 32 patients with bladder cancer.The correlation between miR-30 b expression and clinical and pathological stages of bladder cancer was studied.External transfection was used to increase the expression level of miR-30 b and the effect of the increased expression levels on biological characteristics such as proliferation and invasion of apoptosis of blander cancer cell line T24 were investigated.Results The expression of miR-30 b in the tumor tissues was significantly lower than that in the tumor-adjacent tissues.There was no significant difference between clinical stage and expression of miR-30 b,but the higher the pathological differentiation was,the higher the expression of miR-30 b was.The viability and invasion number of the miR-30 b transfected T24 cell within 48 h was significantly lower than those in the negative control group( P < 0. 05).Conclusion miR-30 b level is related to the occurrence and development of bladder cancer.miR-30 b can significantly inhibit the invasion and accelerate the apoptosis of bladder cancer cells.

关 键 词：MICRORNA 膀胱癌 临床意义 生物学特性 

分 类 号：R737.14[医药卫生—肿瘤]