miR-140-3p通过靶向PD-L1抑制非小细胞肺癌A549细胞的活力、迁移和侵袭  

miR-140-3p inhibits viability,invasion and migration of non-small-cell lung cancer A549 cells by targeting PD-L1

作　　者：张宇轩 李春伟[2] 毛文浩 朱恪岩 邵扬谦 邓晓明[1] ZHANG Yu-xuan;LI Chun-wei;MAO Wen-hao;ZHU Ke-yan;SHAO Yang-qian;DENG Xiao-ming(Department of Combination of TCM and Western Medicine,The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China;Department of Oncology,The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China;Department of Thyroid,Head and Neck Surgery,Henan Cancer Hospital,Zhengzhou 450008,China)

机构地区：[1]郑州大学第一附属医院中西医结合科,河南郑州450052 [2]郑州大学第一附属医院肿瘤科,河南郑州450052 [3]河南省肿瘤医院甲状腺头颈外科,河南郑州450008

出　　处：《中国病理生理杂志》2019年第1期8-14,共7页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.81473497)

摘　　要：目的:探究微小RNA-140-3p(mi R-140-3p)对程序性细胞死亡配体1(PD-L1)的靶向关系以及对非小细胞肺癌A549细胞的活力、迁移和侵袭的影响。方法:使用RT-qPCR检测HLF-1、A549和H1299不同细胞株中mi R-140-3p的表达水平,选择差异最显著的A549细胞用作后续研究对象; Target Scan软件预测和双萤光素酶报告基因实验验证mi R-140-3p和PD-L1之间的靶向关系; RT-qPCR和Western blot检测转染mi R-140-3p模拟物和抑制剂对PD-L1表达水平的影响; MTT法检测mi R-140-3p和PD-L1对A549细胞活力的影响; Transwell实验检测mi R-140-3p和PD-L1对A549细胞迁移和侵袭的影响。结果:mi R-140-3p在人肺癌A549和H1299细胞的表达中显著下调(P <0. 05); mi R-140-3p高表达能够使PD-L1表达下调,对A549细胞的活力、迁移和侵袭具有抑制作用;转染pc DNA3. 0-PD-L1能够阻断mi R-140-3p对A549细胞活力、迁移和侵袭的抑制作用。结论:mi R-140-3p可通过靶向负调控PD-L1抑制A549细胞的活力、迁移和侵袭。AIM:To explore the target relationship between microRNA-140-3p(miR-140-3p)and programmed cell death ligand 1(PD-L1)and their effect on the viability,migration and invasion of non-small-cell lung cancer A549 cells.METHODS:RT-qPCR was used to detect the miR-140-3p expression in HLF-1,A549 and H1299 cells,and then the A549 cells with the most significant difference were selected as the subsequent research object.TargetScan software and dual-luciferase reporter assay were performed to predict and confirm the target relationship between miR-140-3p and PD-L1.RT-qPCR and Western blot were used to determine the effects of miR-140-3p mimic and inhibitor on PD-L1 expression level.MTT assay was used to detect the viability of A549 cells.Transwell assay was performed to detect the migration and invasion abilities of the A549 cells.RESULTS:miR-140-3p was significantly down-regulated in the A549 cells and H1299 cells(P<0.05).Transfection with miR-140-3p mimic decreased the expression of PD-L1 and inhibited the viability,migration and invasion of the A549 cells.Transfection with pcDNA3.0-PD-L1 reversed the inhibitory effect of miR-140-3p on the viability,migration and invasion of the A549 cells.CONCLUSION:miR-140-3p inhibits the viability,migration and invasion of A549 cells by targeting PD-L1.

关 键 词：微小RNA-140-3p 程序性细胞死亡配体1 非小细胞肺癌 

分 类 号：R734.2[医药卫生—肿瘤] R730.23[医药卫生—临床医学]