FBXO5在结直肠癌中的表达及临床意义  

Expression and clinical significance of FBXO5 in colorectal cancer

作　　者：周启明[1,2] 赵艳 付晓红[1,2] 段江曼 康文全[1] ZHOU Qi-ming;ZHAO Yan;FU Xiao-hong;DUAN Jiang-man;KAN Wen-quan(Hospital of Shenzhen Nanshan District,Shenzhen 518052,China;Nanshan Center,Shenzhen Hospital,Cancer Hospital Chinese Academy of Medical Science,Shenzhen 518052,China)

机构地区：[1]深圳市南山区人民医院肿瘤科,518052 [2]中国医学科学院肿瘤医院深圳医院南山肿瘤中心,518052

出　　处：《现代消化及介入诊疗》2018年第6期689-693,共5页Modern Interventional Diagnosis and Treatment in Gastroenterology

基　　金：广东省医学科学技术研究基金(B2016043)

摘　　要：目的探讨FBXO5在结直肠癌中的表达及临床意义。方法采用RT-PCR和Western blot方法检测癌和癌旁组织配对的结直肠癌临床标本中的FBXO5 mRNA和蛋白的表达水平,采用免疫组织化学技术检测240例石蜡包埋的结直肠癌标本,统计学分析FBXO5表达与临床病理学指标、预后的关系。结果与相应配对的癌旁组织相比,癌组织标本中FBXO5 mRNA和蛋白明显上调。统计学分析结果显示,FBXO5表达与结直肠癌患者的临床病理特征密切相关:临床分期(P=0. 004),T分期(P=0. 009),M分期(P=0. 003)。进一步的多因素分析显示,FBXO5表达可作为结直肠癌独立的预后因素(P=0. 045,HR 1. 53,95%CI 1. 01～2. 31)。结论 FBXO5在结直肠癌中表达升高预示结直肠癌患者的不良预后。FBXO5可以成为判断结直肠癌预后的一个新指标。Objective The purpose of this study was to investigate the expression and prognostic significance of FBXO5 in colorectal cancer.Methods RT-PCR and Western blot assays were performed to examine FBXO5 expression in CRC tissues and matched adjacent non-cancerous tissues.And immunohistochemistry was used to evaluate FBXO5 expression in archived 240 paraffin-embedded CRC specimens.Statistical analysis was conducted the relationships between FBXO5 expression and clinicopathologic parameters and prognosis of CRC.Results The mRNA and protein levels of FBXO5 were up-regulated in CRC tissues compared with their adjacent non-cancerous tissues.The FBXO5 expression was closely correlated with clinicopathologic parameters of CRC clinical stage P=0.004 T classification P=0.009 M classification P=0.003.Furthermore multivariate analysis demonstrated that FBXO5 expression was an independent prognostic factor for CRC P=0.045 HR 1.53 95%CI 1.01-2.31.Conclusion FBXO5 level dyregulated and increased in colorectal cancer.High expression of FBXO5 indicated poor prognosis of CRC.FBXO5 may serve as a novel prognostic biomarker for CRC.

关 键 词：结直肠癌 FBX05 预后 

分 类 号：R735.34[医药卫生—肿瘤]