Janus蛋白酪氨酸激酶2/信号转导和转录激活因子3信号转导通路在人慢性根尖周炎中的表达  

Expression of JAK2 and STAT3 in Chronic Periapical Periodontitis

作　　者：张晨[1,2] 刘珺[3] 赵涵[4] 刘玲霜 ZHANG Chen;LIU Jun;ZHAO Han;LIU Ling-shuang(Department of Stomatology, Affiliated Hospital, Qingdao University, Qingdao 266000, China;Qingdao University, Qingdao 266071, China;Department of Orthodontics, Affiliated Hospital of Qingdao University, Qingdao 266003, China;Department of Pathology, Affiliated Hospital of Qingdao University, Qingdao, 266555, China.)

机构地区：[1]青岛大学附属医院,山东青岛266003 [2]青岛大学,山东青岛266071 [3]青岛大学附属医院口腔正畸科,山东青岛266003 [4]青岛大学附属医院病理科,山东青岛266555

出　　处：《口腔医学研究》2019年第1期84-88,共5页Journal of Oral Science Research

基　　金：国家自然科学基金(编号:81500834)

摘　　要：目的:检测Janus蛋白酪氨酸激酶2/信号转导和转录激活子3(Janus kinase 2/signal transducer and activator of transcription 3,JAK2/STAT3)信号转导通路在人慢性根尖周炎中的表达并推测其在慢性根尖周炎中的作用,为研究慢性根尖周病的致病机制提供理论基础。方法:选取健康牙齿牙周膜为对照组,患有根尖周囊肿及根尖周肉芽肿标本分别为实验组1、实验组2,每组各20例,对各组标本分别进行苏木精-伊红(hematoxylin-eosin staining,HE)染色并采用免疫组织化学法检测JAK2、磷酸化Janus蛋白酪氨酸激酶2(phosphorylation-janus kinase 2,p-JAK2)、STAT3及磷酸化信号转导和转录激活子3(phosphorylation-signal transducer and activator of transcription 3,p-STAT3)在各组中的表达。结果:JAK2、p-JAK2、STAT3、p-STAT3在正常牙周组织中均有少量表达,在根尖周囊肿及肉芽肿组织中表达量增加,实验组1、实验组2与对照组比较差异均有统计学意义(P<0.05)。实验组1与实验组2比较,JAK2及p-JAK2的表达差异有统计学意义(P<0.05),STAT3及p-STAT3的表达差异无统计学意义(P>0.05)。对根尖周炎标本中的JAK2和STAT3以及p-JAK2和p-STAT3进行相关性分析,结果显示JAK2和STAT3以及p-JAK2和p-STAT3之间有相关性。结论:JAK2、p-JAK2、STAT3及p-STAT3在慢性根尖周炎中表达量增加,推测JAK2/STAT3信号通路与根尖周炎的炎症过程有关,可能在其发展过程中有重要意义。Objective: To detect the expression of JAK2/STAT3 signal transduction pathway in chronic apical periodontitis in human and speculate its role in chronic periapical diseases.Methods: Twenty healthy periodontal membranes,20 periapical cysts,and 20 periapical granuloma specimens were stained by HE.Immunohistochemistry was used to detect the expression of JAK2,p-JAK2,STAT3,and p-STAT3 in different groups.Results: JAK2,p-JAK2,STAT3,and p-STAT3 were slightly expressed in normal tissues,and increased in periapical cysts and granuloma.The differences were statistically significant (P<0.05).The difference between JAK2 and p-JAK2 was statistically significant (P<0.05).There was no significant difference in STAT3 and p-STAT3 between periapical cyst group and periapical granuloma group (P>0.05).Tere was a correlation between JAK2 and STAT3 and p-JAK2 and p-STAT3.Conclusion: The expressions of JAK2,p-JAK2,STAT3,and p-STAT3 were increased in chronic periapical diseases.We speculate that JAK2/STAT3 signaling pathway is involved in the inflammatory process of periapical perioditis and may play an important role in its development.

关 键 词：Janus蛋白酪氨酸激酶2 信号转导和转录激活子3 根尖周囊肿 根尖周肉芽肿 

分 类 号：R781.341[医药卫生—口腔医学]