hMTERF3与C-met在胃癌中的表达及临床意义  

Expression and Clinical Significance of hMTERF3 and C-met in Gastric Cancer

作　　者：李戈 李中福[1] 王灿[1] 韩继明[1] 马莎英 LI Ge;LI Zhongfu;WANG Can;HAN Jiming;MA Shaying(Department of General Surgery,Chongqing Emergency Medical Center/Chongqing Fourth People's Hospital/Affiliated CentralHospital of Chongqing University,Chongqing,400014,China)

机构地区：[1]重庆市急救医疗中心/重庆市第四人民医院/重庆大学附属中心医院普外科,重庆400014

出　　处：《肿瘤药学》2018年第5期701-704,723,共5页Anti-Tumor Pharmacy

基　　金：重庆市永川区2015年联合资助科技计划项目(Ycstc;2015nc5022)

摘　　要：目的探讨人线粒体转录终止因子(hMTERF3)与C-met在胃癌组织中的表达变化及其临床意义。方法采用SP免疫组化方法检测hMTERF3与C-met在胃癌组织和正常胃黏膜组织中的表达;RT-PCR法检测hMTERF3与C-met mRNA表达,蛋白免疫印迹法(Western blot)检测蛋白表达。结果胃癌组织和正常胃黏膜组织中均可检测到hMTERF3和C-met的表达,hMTERF3阳性率分别为89.66%和27.59%,C-met阳性率分别为81.61%和17.24%,差异均有统计学意义(P<0.01)。hMTERF3和C-met在胃癌组织中的蛋白及mRNA表达量明显高于正常胃黏膜组织,差异均有统计学意义(P<0.01)。胃癌组织中hMTERF3和C-met蛋白表达呈正相关(r=0.419,P<0.05)。hMTERF3和C-met蛋白阴性表达的患者5年内生存率显著高于阳性表达患者,差异有统计学意义(P<0.05)。结论胃癌组织中hMTERF3和C-met的表达均明显上调,并表现出协同效应。生存分析显示两种蛋白的阳性表达与胃癌患者的预后不良相关。Objective To study the expression of human mitochondrial transcription termination factor 3(hMTERF3)and C-met in gastric cancer tissues and the relationships with prognosis of gastric cancer.Methods SP immunohistochemistry was used to detect the expressions of hMTERF3 and C-met in gastric cancer tissues and normal gastric mucosa tissues.RT-PCR was applied to detect the mRNA expression of hMTERF3 and C-met,and Western blot was applied to detect the protein expression of hMTERF3 and C-met.Results The expressions of hMTERF3 and C-met can be detected in both gastric cancer tissue and normal gastric mucosa tissue.The positive rate of hMTERF3 was respectively 89.66% and 27.59%,and that of C-met was 81.61% and 17.24% respectively.All had significant differences(P<0.01).The mRNA and protein expression levels of hMTERF3 and C-met were all detected higher in gastric cancer tissues than in normal gastric mucosa tissues(P<0.05).In the gastric cancer tissues,the expression of hMTERF3 protein was positively correlated with the expression of C-met protein(Spearman correlation coefficient r=0.419,P<0.05).Patients with negative expressions of hMTERF3 and C-met protein had significantly higher five-year survival rate than those with the positive expressions of hMTERF3 and C-met protein(P<0.05).Conclusion The expression levels of hMTERF3 and C-met was obviously up-regulated in gastric cancer tissues and showed synergistic effect in gene and protein level.Survival analysis showed that positive overexpression of the two proteins was associated with poor prognosis in patients with gastric cancer.

关 键 词：胃癌 hMTERF3 C-MET 预后 

分 类 号：R735.2[医药卫生—肿瘤]