无铜离子洗脱预染纸色谱-分光光度法定量γ-氨基丁酸  

No-Cu^(2+) elution pre-staining paper chromatography-vis-spectrophotometry for quantification of GABA

作　　者：陈圆洪 常坤朋 李海星[4,3] 聂丽娟 王水兴[1,3] 刘晓华 CHEN Yuanhong;CHANG Kunpeng;LI Haixing;NIE Lijuan;WANG Shuixing;LIU Xiaohua(Department of Environmental Resources and Chemical Engineering,Nanchang University,Nanchang 330047,China;Key Laboratory of Poyang Lake Environment and Bio-Resources Utilization,Ministry of Education China,Nanchang University,Nanchang 330047,China;State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China;Sino-German Joint Research Institute,Nanchang University,Nanchang 330047,China)

机构地区：[1]南昌大学资源环境与化工学院,江西南昌330047 [2]南昌大学鄱阳湖环境与资源利用教育部重点实验室,江西南昌330047 [3]南昌大学中德联合研究院,江西南昌330047 [4]南昌大学食品科学与技术国家重点实验室

出　　处：《南昌大学学报（理科版）》2018年第5期456-460,共5页Journal of Nanchang University(Natural Science)

基　　金：国家自然科学基金资助项目(21566023;31570070)

摘　　要：γ-氨基丁酸(Gamma-aminobutyric acid,GABA)在食品和医药领域具有重要作用。许多方法已用于测定GABA,包括以Cu2+为稳定剂的预染纸色谱-分光光度法。本论文报道了一种新的GABA定量方法:无Cu2+洗脱预染纸色谱-分光光度法(No-Cu2+elution pre-staining paper chromatography-vis-spectrophotometry,NCE-PSPCVS)。该方法是将滤纸中的GABA斑点剪下,每个斑点用5mL 75%的乙醇溶液洗脱,待GABA完全洗脱后,用紫外分光光度计测定洗脱液在570nm处的光吸收值(A570)。此方法准确性高,线性关系良好,R2为0.994,相对标准偏差RSD为0.82%～1.72%,回收率96.95%～102.90%。经高效液相色谱法(HPLC)验证,此方法准确性高,可用于GABA定量测定。Gamma-aminobutyric acid plays an important role in the fields of food and pharmaceutics.Many methods have been employed to determine gamma-aminobutyric acid,including pre-staining paper chromatography-vis-spectrophotometry in which Cu^2+was described to stabilize ninhydrin-ninhydrin hydrate chromophore.In the current work,we report a new quantitative method to measure gamma-aminobutyric acid,termed as No-Cu^2+elution pre-staining paper chromatography-vis-spectrophotometry(NCE-PSPC-VS).The GABA spots were cut out from the paper and were eluted with 5mL 75%ethanol solutions.Afterwards the absorption values at 570 nm(A 570)of the eluents were read in a UV-vis spectrophotometer.This method is accurate with R^2=0.994,RSD ranging from 0.82%-1.72%,and recoveries between 96.95%-102.90%.The validation of this method was confirmed by HPLC,indicating that the developed approach could be an alternative of xisting methods for the determination of gamma-aminobutyric acid.

关 键 词：纸色谱 可见分光光度法 无Cu^2+洗脱 γ-氨基丁酸(GABA) 

分 类 号：Q963[生物学—昆虫学]