星点设计-响应面法优化超声提取脱皮马勃粗多糖工艺  

Optimization of Ultrasound-assisted Crude Polysaccharide Extraction from Lasiosphaera Fenzlii by Central Composite Design-Response Surface Methodology

作　　者：史伟国[1] 白国栋 宗希明[1] 袁寰[1] 杜鹃[1] 高庆东[2] Shi Weiguo;Bai Guodong;Zong Ximing;Yuan Huan;Du Juan;Gao Qingdong(College of Pharmacy,Jiamusi University,Jiamusi 154007,China;Chinese TraditionalMedicine Hospital of Jiamusi,Jiamusi 154002,China)

机构地区：[1]佳木斯大学药学院,黑龙江佳木斯154007 [2]佳木斯市中医医院,黑龙江佳木斯154002

出　　处：《中国野生植物资源》2018年第5期4-7,共4页Chinese Wild Plant Resources

基　　金：林业公益性行业科研专项子项目(2010040401-02);黑龙江省教育厅科学技术研究项目(12511577);佳木斯大学科学技术研究重点项目(Sz2011-013)

摘　　要：药用真菌马勃中含甾体、萜类、多糖等多种成分,其中多糖是其主要活性成分之一。采用星点设计-响应面法优化超声辅助提取马勃粗多糖的最佳工艺为:液料比30. 00 ml·g^(-1),提取时间53. 77 min,提取温度58. 33℃,马勃粗多糖的提取率为:4. 01%。使用该方法优化的马勃粗多糖提取工艺具有操作简单、合理、预测性好的优点。可以为马勃的开发利用奠定基础,提供基础数据。Medicinal fungi puffball contains a variety of ingredients such as steroid,terpenoids and polysaccharide,of which polysaccharide is one of the main active ingredient.The optimization of ultrasound assisted extraction of optimum puffball polysaccharide for the central composite design response surface method:liquid-to-solid ratio 30∶1,relflux time 53.77 min,extraction temperature 58.33℃,the extraction rate of puffball curde polysaccharide was 4.01%.This method is used to optimize the extraction process of puffball crude polysaccharide with the advantages of simple operation,reasonable and predictive.It can lay the foundation for the development and utilization of puffball and provide basic data.

关 键 词：脱皮马勃 粗多糖 超声提取 星点设计 响应面法 

分 类 号：S567[农业科学—中草药栽培]