CEACAM-1在结直肠癌中的表达及与肿瘤血管生成、转移的相关性研究  

Expression of CEACAM-1 in colorectal carcinoma and correlation with tumor angiogenesis and metastasis

作　　者：夏龙飞 刘玉君 张军民 韩俊毅[2] XIA Longfei;LIU Yujun;ZHANG Junmin;HAN Junyi(Department of General Surgery,Maternal and Child Health Hospital of Zhoushan City,Zhejiang Province,Zhoushan 316000,China;Department of Gastrosurgery,Dongfang Hospital,Tongji University,Shanghai 200120,China)

机构地区：[1]浙江省舟山市妇幼保健院普外科,浙江舟山316000 [2]同济大学附属东方医院胃肠外科,上海200120

出　　处：《中国医药导报》2019年第2期94-97,183,共5页China Medical Herald

摘　　要：目的探讨癌胚抗原相关细胞黏附分子1(CEACAM-1)在结直肠癌中的表达及与肿瘤血管生成、转移之间关系,探讨其作为结直肠癌的诊断、治疗及预后评价监测指标的可行性。方法收集2016年1月～2017年1月舟山市妇幼保健院普外科手术切除的50例结直肠癌组织和50例正常结直肠组织,用免疫组化法检测CEACAM-1和血管内皮生长因子(VEGF)的表达情况,同时观察微血管密度值(MVD),分析三者与肿瘤临床病理特征之间的关系及三者之间的相关性。结果 CEACAM-1和VEGF在结直肠癌组织中阳性表达明显高于正常组织中的表达,差异有高度统计学意义(P=0.000);结直肠癌组织中MVD值明显高于正常组织,差异有高度统计学意义(P=0.000);结直肠癌组织中CEACAM-1、VEGF的表达和MVD值均与结直肠肿瘤分化程度、浆膜浸润、TNM分期、脉管浸润、淋巴结转移及肝转移相关(P <0.05);结直肠癌组织中CEACAM-1和VEGF阳性表达组中MVD值显著高于阴性组(P <0.01);CEACAM-1与VEGF在结直肠癌组织中的表达呈正相关性(r=0.483,P <0.001)。结论 CEACAM-1高表达能诱导肿瘤微血管的生成,与结直肠癌的浸润及血行转移关系密切,CEACAM-1可作为结直肠癌早期诊断、预后监测的潜在分子生物学指标。Objective To investigate the expression of CEACAM-1 and the correlation with the tumor angiogenesis and metastasis,and explore feasibility as a monitoring indicator for the diagnosis,treatment and prognosis in evaluating colorectal carcinoma.Methods Fifty specimens of colorectal adenomacarcinoma tissues and 50 specimens of normal colorectal tissues from January 2016 to January 2017 in the Department of General Surgery,Maternal and Child Health Hospital of Zhoushan City were collected.The expression of CEACAM-1,VEGF was detected by immunohistochemistry method,and the value of micro-vascular density(MVD)was observed.The correlation between clinicopathologic features of tumors and expression of CEACAM-1,VEGF and MVD value,as well as the correlation among the three indices was analyzed.Results The positive expression of CEACAM-1 and VEGF in colorectal carcinoma tissues were significantly higher than those in normal tissues,and the differences were highly significant(P=0.000);MVD value in colorectal carcinoma tissues was significantly higher than that of normal tissue,and the difference was highly significant(P=0.000);the expression of CEACAM-1,VEGF and MVD value were correlated to degree of colorectal tumor differentiation,tumor serous membrane infiltration,TNM staging,vascular infiltration,lymph node metastasis and liver metastasis(P﹤0.05);MVD value of CEACAM-1 and VEGF positive expression groups was significantly higher than that of the negative group in colorectal carcinoma tissues(P﹤0.01);the expression of CEACAM-1 and VEGF were positively correlated in colorectal carcinoma tissues(r=0.483,P﹤0.001).Conclusion The high expression of CEACAM-1 can induce the formation of tumor microvasculature,and is closely related to the tumor infiltration and metastasis.CEACAM-1 can be used as a potential molecular biological indicator for early diagnosis and prognosis in monitoring colorectal carcinoma.

关 键 词：结直肠癌 癌胚抗原相关细胞黏附分子-1 血管内皮生长因子 微血管密度 相关性 

分 类 号：R735.37[医药卫生—肿瘤] R735.35[医药卫生—临床医学]