细胞因子FAM3C在携带人突变型α-突触核蛋白A53T纯合子小鼠黑质区表达的研究  

EXPRESSION OF THE CYTOKINE FAM3CIN THE SUBSTANTIA NIGRA OF HOMOZYGOUS MICE CARRYING A53T HUMAN α-SYNUCLEIN

作　　者：张培[1] 焦倩[1] 杜希恂[1] 姜宏[1] ZHANG Pei;JIAO Qian;DU Xixun;JIANG Hong(State Key Disciplines: Physioloy(in Incubation), Department of Physiology, Qingdao University, Qingdao 266071, China)

机构地区：[1]青岛大学国家生理学重点(培育)学科,山东青岛266071

出　　处：《精准医学杂志》2018年第6期542-545,共4页Journal of Precision Medicine

基　　金：国家自然科学基金项目(31771110);国家自然科学基金(青年基金)项目(31500837)

摘　　要：目的探讨细胞因子FAM3家族成员FAM3C在帕金森病(PD)模型小鼠黑质(SN)区的表达及其意义。方法选取3及6月龄携带人突变型α-突触核蛋白A53T纯合子(α-SynA53T^(+/+))小鼠和同窝野生型(WT)小鼠,提取SN区蛋白,采用Western Blot方法检测FAM3C蛋白的表达水平。结果 3月龄α-SynA53T^(+/+)小鼠SN区FAM3C蛋白表达水平与WT小鼠相比差异无显著意义(t=1.245,P>0.05),6月龄α-SynA53T^(+/+)小鼠SN区FAM3C蛋白表达水平与WT小鼠相比明显升高,差异有统计学意义(t=8.865,P<0.05)。结论 3月龄小鼠SN区FAM3C蛋白表达水平不变,而6月龄小鼠SN区FAM3C蛋白表达水平升高,说明SN区FAM3C蛋白随PD病情进展表达增加,可能参与了PD的发病过程。Objective To investigate the expression of FAM3C,a member of the cytokine FAM3 family,in the substantia nigra (SN) of mice with Parkinson’s disease (PD) and its clinical significance.Methods The homozygous mice carrying A53T human α-synuclein (α-SynA53T +/+ mice),aged 3 and 6 months,and their littermate wild-type (WT) mice were selected.The protein in the SN was extracted,and Western Blot was used to measure the protein expression of FAM3C.ResultsThere was no significant difference in the expression of FAM3C protein in the SN between the α-SynA53T +/+ mice aged 3 months and the WT mice (t=1.245,P>0.05).Compared with the WT mice,the α-SynA53T +/+ mice aged 6 months had significantly higher expression of FAM3C protein in the SN (t=8.865,P<0.05).ConclusionThe expression of FAM3C protein in the SN has no change in mice aged 3 months,but shows an increase in mice aged 6 months,suggesting that the expression of FAM3C in the SN increases with the progression of PD and that FAM3C may be involved in the pathogenesis of PD.

关 键 词：FAM3C蛋白 黑质 帕金森病 疾病模型 动物 

分 类 号：R742.5[医药卫生—神经病学与精神病学]