miR-152在急性髓系白血病患者骨髓和细胞系中的表达及生物学功能的初步研究  

Preliminary study on expression and biological functions of miR-152 in acute myeloid leukemia patients bone marrow and cell lines

作　　者：刘文霞 王晓雪[2] 李艳[2] Liu Wenxia;Wang Xiaoxue;Li Yan(Department of Hematology,the Central Hospital of Yingkou,Liaoning Yingkou 115000,China;Department of Hematology,the First Affiliated Hospital of China Medical University,Liaoning Shenyang 110001,China)

机构地区：[1]营口市中心医院血液科,辽宁营口115000 [2]中国医科大学附属第一医院血液科,辽宁沈阳110001

出　　处：《现代肿瘤医学》2019年第3期376-381,共6页Journal of Modern Oncology

基　　金：辽宁省科技厅课题(编号:2013225079)

摘　　要：目的:检测miR-152在急性髓系白血病(AML)患者骨髓和细胞系中的表达水平。初步研究miR-152在急性髓系白血病中的生物学功能。方法:收集急性髓系白血病患者40例,非恶性血液病对照组20例,提取骨髓有核细胞;培养U937、Kasumi-1、THP-1三种急性髓系白血病细胞系。RT-PCR检测miR-152的表达水平。分别对U937细胞系和Kasumi-1细胞系转染miR-152 mimics和inhibitor,CCK-8法检测U937细胞及Kasumi-1细胞增殖情况,Annexin V/PI流式实验检测细胞凋亡,流式细胞术检测细胞周期。结果:miR-152在急性髓系白血病患者骨髓及细胞系中较非恶性血液病对照组表达明显下降。U937细胞系转染miR-152 mimics后,增殖受抑制,细胞凋亡增加,细胞阻滞在G_0-G_1期; Kasumi-1细胞系转染miR-152inhibitor后,增殖增加,细胞凋亡减少,G_0-G_1期细胞减少。结论:miR-152在急性髓系白血病患者骨髓及细胞系中的表达水平均较正常对照组显著降低。miR-152在急性髓系白血病中起抑癌基因的作用,过表达miR-152可以抑制细胞增殖,促进凋亡。Objective:To detect the expression level of miR-152 in acute myeloid leukemia patients bone marrow and cell lines.A preliminary study on the biological function of miR-152 in AML was also conducted.Methods:40 patients with AML and 20 cases with non-malignant blood disease were collected.AML cell lines U937,THP-1 and Kasumi-1 were cultured.Expression level of miR-152 was detected by RT-PCR.miR-152 mimics/inhibitor respectively transfected U937 cell lines and Kasumi-1 cell lines.U937 cell and Kasumi-1 cell proliferation were detected by CCK-8 method.Flow cytometry was used to detect cell apoptosis and cell cycle.Results:miR-152 was significantly decreased in AML patients bone marrow and cell lines compared with non-malignant hematologic disease control group.miR-152 mimics were transfected into U937 cell line.U937 cells were blocked in G 0-G 1 phase,and proliferation was inhibited and apoptosis was increased after transfection.miR-152 inhibitor were transfected into Kasumi-1 cell lines,showing that cells in G 0-G 1 were reduced,and proliferation was increased and apoptosis was decreased.Conclusion:Expression of miR-152 was significantly lower in AML patients bone marrow and cell lines than normal control.miR-152 plays a role of tumor suppressor genes in AML.Over expression of miR-152 can inhibit cell proliferation,promote apoptosis.

关 键 词：miR-152 急性髓系白血病 增殖 凋亡 细胞周期 

分 类 号：R733.7[医药卫生—肿瘤]