HDAC2在肝细胞肝癌中对MMP9表达的影响  

The impact of HDAC2 on MMP9 expression in hepatocellular carcinoma

作　　者：尹中波 王春玉[2] 赵越[2] Yin Zhongbo;Wang Chunyu;Zhao Yue(Department of Pathology,the Sixth People's Hospital of Nantong,Jiangsu Nantong 226011,China;Department of Cell Biology,Key Laboratory of Cell Biology,China Medical University,Liaoning Shenyang 110001,China)

机构地区：[1]南通市第六人民医院病理科,江苏南通226011 [2]中国医科大学基础医学院染色质生物学研究室.教育部医学细胞生物学重点实验室.卫生部细胞生物学重点实验室,辽宁沈阳110001

出　　处：《现代肿瘤医学》2019年第3期390-393,共4页Journal of Modern Oncology

基　　金：国家自然科学基金(编号:31401115)

摘　　要：目的:明确组蛋白去乙酰化酶2(HDAC2)在肝细胞肝癌(HCC)中对基质金属蛋白酶9(MMP9)表达的影响。方法:利用mRNA表达谱芯片数据进行统计分析,确定HDAC2和MMP9的mRNA在HCC组织中的表达情况及相关性;实时定量PCR检测HDAC2过表达对MMP9转录的影响;蛋白免疫印迹检测HDAC2过表达对MMP9蛋白表达的影响。结果:HDAC2和MMP9的mRNA在HCC肿瘤组织中均表达升高并呈正相关性;在Hep G2细胞中,HDAC2促进MMP9的转录及蛋白表达。结论:HDAC2在HCC中促进MMP9的表达,可能在HCC转移过程中发挥作用。Objective:To evaluate the impact of HDAC2 on MMP9 expression in hepatocellular carcinoma(HCC).Methods:The expressions of HDAC2 and MMP9 in HCC tissues were evaluated with microarray data,and their correlation was analyzed.Real-time PCR was performed to detect the effect of HDAC2 overexpression on mRNA level of MMP9.Western Blot was used to detect the effect of HDAC2 overexpression on protein level of MMP9.Results:Both HDAC2 and MMP9 were higher expressed in HCC tissues,compared with that in normal liver tissues.There was a positive correlation between HDAC2 expression and MMP9 expression.In HepG2 cells,overexpression of HDAC2 increased both mRNA and protein expression of MMP9.Conclusion:HDAC2 promotes MMP9 expression in HCC,which may be involved in tumor metastasis of HCC.

关 键 词：组蛋白去乙酰化酶2 基质金属蛋白酶9 肝细胞肝癌 肿瘤转移 

分 类 号：R735.7[医药卫生—肿瘤]