一株深海热液口嗜热芽孢杆菌SY27F代谢产物活性的研究  

Evaluating bioactivity of metabolites of a hydrothermal Bacillus sp. SY27F from the deep-sea hydrothermal vent

作　　者：周洪[1] 张勤[1] 何聿 杨黄浩[1,2] 田永奇 Hong Zhou;Qin Zhang;Yu He;Huanghao Yang;Yongqi Tian(College of Biological Science and Engineering,Fuzhou University,Fuzhou 350116,Fujian Province,China;MOE Key Laboratory for Analytical Science of Food Safety and Biology,State Key Laboratory of Photocatalysis on Energy and Environment,College of Chemistry,Fuzhou University,Fuzhou 350116,Fujian Province,China)

机构地区：[1]福州大学生命科学与工程学院,福建福州350116 [2]福州大学化学学院国家食品安全与生物分析科学实验室国家能源与环境光催化重点实验室,福建福州350116

出　　处：《微生物学报》2019年第2期314-325,共12页Acta Microbiologica Sinica

基　　金：福州大学科研启动基金(XRC-1672;2017T022);福州市科技局项目(2017-N-39);福建省教育厅项目(JAT170077);中科院热带海洋生物资源和生态学重点实验室开放课题(2018011009);广东省海洋药物重点实验室(LMM2018-3)~~

摘　　要：【目的】对一株来源于深海热液口嗜热芽孢杆菌的次生代谢产物进行抑菌活性和抗肿瘤活性的初步研究。【方法】采用纸片法和微量肉汤稀释法检测嗜热芽孢杆菌SY27F次生代谢产物的抑菌活性,采用CCK-8法测定其次生代谢产物的抗肿瘤活性。【结果】抑菌实验表明,嗜热芽孢杆菌代谢产物对大肠杆菌、金黄色葡萄球菌均有抑菌作用,其最低抑菌浓度分别为1.56 mg/mL和3.13 mg/mL;细胞实验表明,其代谢产物对肿瘤细胞A549、HepG2、HeLa、MCF-7均有一定的抑制作用,其半致死浓度分别为0.390、0.451、0.704、1.105 mg/mL;与人肝肿瘤细胞(HepG2)相比,其对人正常肝细胞(L02)表现出良好的生物相容性。【结论】嗜热芽孢杆菌SY27F次生代谢产物具有一定的抑菌和抗肿瘤活性,可为寻找新型抑菌抗肿瘤活性物质提供优质资源。[Objective] Extracting the metabolites of hydrothermal Bacillus sp. SY27F from deep-sea hydrothermal vents, the antibacterial and antitumor activity of metabolites were assayed. [Methods] The antibacterial activity of the secondary metabolites was determined by the method of paper disk and microbroth dilution method, and the anti-tumor activity of the secondary metabolites was evaluated by CCK-8 method. [Results] The bacteriostatic experiments showed that the metabolites of thermophilic Bacillus sp. SY27F have antibacterial effect onEscherichia coli and Staphylococcus aureus, with MICs of 1.56 mg/mL and 3.13 mg/mL, respectively. Cell cytotoxicity assay showed that the metabolites have the effect of inhibiting cell growth in tumor cells（A549, HepG2, HeLa and MCF-7）, with IC50 values of 0.390 mg/mL, 0.451 mg/mL, 0.704 mg/mL and 1.105 mg/mL, respectively. Compared with HepG2, the normal liver cells（L02） showed good biocompatibility. [Conclusion] The metabolites of thermophilic Bacillus sp. SY27F have antibacterial and antitumor activity, and can provide resources for the search of novel compound of antibacterial and antitumor activity.

关 键 词：深海热液口 嗜热芽孢杆菌 代谢产物 抑菌活性 抗肿瘤活性 

分 类 号：R915[医药卫生—微生物与生化药学]