响应面分析法优化竹叶蛋白质提取工艺  

Optimization of Extraction Process for Protein from Bamboo Leaves by Response Surface Methodology

作　　者：范煜[1] 王鑫宁 张强 蒋立新 吴良如[2] 陈颖 何经晟 肖竹钱 葛青[1] 毛建卫[1] 胡林福 FAN Yu;WANG Xing-ning;ZHANG Qiang;JIANG Li-xin;WU Liang-ru;CHEN Ying;HE Jing-sheng;XIAO Zhu-qian;GE Qing;MAO Jian-wei;HU Lin-fu(Zhejiang Provincial Key Lab for Chem & Bio Processing Technology of Farm Product, Zhejiang Provincial CollaborativeInnovation Center of Agricultural Biological Resources Biochemical Manufacturing, School of Biological and ChemicalEngineering, Zhejiang University of Science and Technology, Hangzhou 310023, Zhejiang, China;China National Bamboo Research Center, Hangzhou 310012, Zhejiang, China;Zhejiang Shengshi Biotechnology Co. LTD, Huzhou 313399, Zhejiang, China)

机构地区：[1]浙江省农产品化学与生物加工技术重点实验室浙江省农业生物资源生化制造协同创新中心浙江科技学院生物与化学工程学院,浙江杭州310023 [2]国家林业局竹子研究开发中心,浙江杭州310012 [3]浙江圣氏生物科技有限公司,浙江湖州313399

出　　处：《竹子学报》2018年第3期37-44,共8页Journal of Bamboo Research

基　　金：浙江省重点研发项目(2015C02031);浙江省农业生物资源生化制造协同创新中心开放基金(2016KF0035;2016KF0110);浙江科技学院2017年研究生创新基金(2017YJSKC018)

摘　　要：以提高竹叶蛋白质得率为优化目标,采用响应面法对竹叶蛋白质提取工艺进行优化,考察提取次数、p H、温度、时间和液料比对竹叶蛋白质提取得率的影响。利用Box-Behnken试验和响应面分析法,研究了各变量及其交互作用对竹叶蛋白质得率的影响,模拟得到二次多项式回归方程的预测模型,得到最佳提取工艺。通过回归分析确定优化后的提取工艺参数为:提取2次,p H 6,温度33. 17℃,时间87.60 min,液料比23. 39 m L·g^(-1),蛋白质得率为7. 64%。此条件下,竹叶蛋白质提取得率的实测结果为7. 49%,与响应面拟合所得方程的预测值符合,比未优化的纯水提取竹叶蛋白得率提高了2. 621%。优化得出的回归模型具有一定的实际指导意义,为竹叶蛋白质提取的工厂放大和深入研究提供一定的实验研究依据。In order to improve the extraction yield of the protein from bamboo leaves, we optimized response surface methodology to investigate the effects of the extraction conditions including pH, temperature, duration and the extract-substrate ratio. According to the single factor experiments, the optimum conditions for extraction of bamboo leaf proteins was forecasted via Box-Benhnken central composite design and response surface methodology. The prediction model of quadratic polynomial regression equation was simulated. The results showed that the optimal conditions for extraction of bamboo leaf protein were as follows: extracted twice at pH 6, 33.17 ℃ for 87.60 min, and extract-substrate ratio of 23.39 mL·g^-1 . Under these conditions the yield of protein amounted to 7.64%. The experimental extraction yield of protein was 7.49%, with the relative error of 0.15% in comparison with the predicted value. This indicated that the optimized regression equation may derive practical significance, providing a theoretical and experimental reference for industrial extraction.

关 键 词：竹叶 蛋白质 提取 响应面法 

分 类 号：TQ936.2[化学工程]