骨肉瘤患者血清血管内皮生长因子及血管生成素-2的表达  

Study on the value of serum vascular endothelial growth factor and angiopoietin-2 expression in elderly patients with osteosarcoma

作　　者：张小军 裴东红[2] 王玉 纪波 Zhang Xiaojun;Pei Donghong;Wang Yu;Ji Bo(Department of Orthopaedics,People's Hospital of Tongchuan,Shaanxi Tongchuan 727000,China;Department of Orthopaedics,People's First Hospital of Yulin,Shaanxi Yulin 718000,China;Department of Physical Examination Center,People's Hospital of Tongchuan,Shaanxi Tongchuan 727000,China)

机构地区：[1]铜川市人民医院骨外科,陕西铜川727000 [2]榆林市第一医院骨外科,陕西榆林718000 [3]铜川市人民医院体检中心,陕西铜川727000

出　　处：《现代肿瘤医学》2019年第4期654-656,共3页Journal of Modern Oncology

基　　金：陕西省社会发展科技攻关项目(编号:2016SF-187)

摘　　要：目的:研究骨肉瘤患者血清血管内皮生长因子(VEGF)、血管生成素-2(Ang-2)表达的动态变化,为临床相关诊治提供参考。方法:回顾性分析铜川市人民医院和榆林市第一医院于2016年1月至2018年1月期间收治的骨肉瘤患者40例作为观察组,同期选取两院参加健康体检的50例健康个体作为对照组。采用酶联免疫吸附法(ELISA法)对比两组患者VEGF、Ang-2含量,并比较肿瘤TNM不同分期、不同肿瘤直径及是否出现肺内转移患者上述指标含量变化。结果:两组间相比,观察组患者血清VEGF、Ang-2含量均高于对照组,差异均有统计学意义(P <0. 05);不同分期相比,Ⅲ期患者血清VEGF、Ang-2含量均高于Ⅱ_b期,而Ⅱ_b期患者高于Ⅱ_a期,差异均有统计学意义(P <0. 05);不同肿瘤直径及转移相比,肿瘤直径≥5 cm患者血清VEGF、Ang-2水平高于肿瘤直径<5 cm者,且出现肺内转移患者血清VEGF、Ang-2水平高于无肺内转移者,差异均有统计学意义(P <0. 05)。结论:骨肉瘤患者血清VEGF、Ang-2含量增高,且随着肿瘤分期增加而升高,且与肿瘤直径及转移呈正相关,可为临床诊治提供重要参考。Objective: To investigate the dynamic changes of serum vascular endothelial growth factor (VEGF) and angiopoietin-2 (Ang-2) expression in elderly patients with osteosarcoma,so as to provide references for clinical diagnosis and treatment. Methods: A retrospective analysis of 40 elderly patients with osteosarcoma admitted in People's Hospital of Tongchuan and People's First Hospital of Yulin from January 2016 to January 2018 was conducted as observation group.Meanwhile,50 healthy individuals who participated in health examination in our two hospitals were selected as control group.Enzyme linked immunosorbent assay (ELISA) was used to compare the contents of VEGF and Ang-2 in the two groups.The changes of these indexes in patients with different stages of TNM,different tumor diameters and whether there were pulmonary metastasis were compared. Results: Compared between the two groups,the observation group patients serum VEGF,Ang-2 levels were higher than the control group,the differences were statistically significant ( P <0.05),different stages compared to serum VEGF,Ang-2 in stage III was higher than that in stage B,and stage B patients was higher than that in stage a,the difference was statistically significant ( P <0.05),compared different tumor size and metastasis,tumor diameter over 5 cm in sera of patients with VEGF,Ang-2 level higher than the tumor diameter less than 5 cm,and the emergence of intrapulmonary metastasis of serum VEGF and Ang-2 levels than those without lung metastasis,there were statistically significant differences( P <0.05). Conclusion: The serum levels of VEGF and Ang-2 in elderly patients with osteosarcoma increased,and increased with the increase of tumor stage,and positively correlated with the diameter and metastasis of tumor.It can provide important reference for clinical diagnosis and treatment.

关 键 词：骨肉瘤 血管内皮生长因子 血管生成素-2 酶联免疫吸附法 

分 类 号：R738.1[医药卫生—肿瘤]